子午流注纳甲法对局灶性脑缺血模型大鼠脑区NSE、NGF、bFGF的影响

发布时间：2018-12-08 13:25

【摘要】：目的:通过制作大鼠大脑中动脉永久性闭塞模型,观察不同时段NSE、NGF、b FGF的表达变化,探究子午流注针法对脑缺血后神经干细胞内源性激活作用及其机制,从而为临床治疗提供科学依据。方法:选用体质量200~250 g的144只Wistar大鼠,按随机数字表法分为正常组、假手术组、手术组、辨证取穴组(简称辨证组)和纳甲法组(简称纳甲组)5大组各45只动物,再将5大组动物根据针刺治疗时间的长短,在各组内再随机分为术后6 h、3、7、14、21 d 5个小组,其中每小组各8只动物。参照Longa法建立大脑中动脉栓塞模型局灶性缺血模型(MCAO)。治疗于实验第2天开始针灸治疗,每日同一时间治疗。常规辨证组参照《实验针灸学》选穴,选取水沟、合谷和三阴交用平补平泻法,每天1次,每次留针30 min。纳甲组根据郑魁山《子午流注与灵龟八法》所记载的徐氏子午流注纳甲法,依日按时开穴针刺治疗,手法与留针时间同辨证组。处死动物观察神经元形态学病理变化,用酶联免疫法(ELISA)检测大脑匀浆中神经元特异性烯醇化酶(NSE)、神经生长因子(NGF)、碱性成纤维生长因子(b FGF)浓度。结果:与正常组比较MCAO组大鼠的神经元结构受损,与正常组比较MCAO大鼠大脑的NSE、NGF、b FGF均呈现先升高再降低的动态表达变化,各治疗组均可抑制NSE的升高,增加NGF、b FGF的含量,其作用效应也呈现出同样的先升高再降低动态表达变化规律,其中子午流注纳甲法组、综合组作用在早期优于常规组。结论:针刺能促进MCAO大鼠海马CA1区神经元结构修复,促进MCAO大鼠缺血侧大脑匀浆NGF、b FGF的表达,抑制NSE的表达,纳甲组、联合组效果优于常规针刺。针刺在缺血早期促进脑缺血后神经干细胞的激活、增殖、分化作用更明显,提示针刺早期干预的作用更加明显。
[Abstract]:Objective: to investigate the endogenous activation of neural stem cells (NSCs) after cerebral ischemia by making a rat model of permanent middle cerebral artery occlusion (MCAO) and observing the changes of NSE,NGF,b FGF expression in different periods. So as to provide a scientific basis for clinical treatment. Methods: one hundred and forty-four Wistar rats with body weight of 200g were randomly divided into normal group, sham operation group, syndrome differentiation group (abbreviated as syndrome differentiation group) and nacha group (nata group). According to the time of acupuncture treatment, the five groups were randomly divided into 5 groups at 6 h after operation, including 8 animals in each group. Establishment of focal Ischemia Model with Longa method in Middle Cerebral artery Embolization Model Acupuncture and moxibustion began on the second day of the experiment and were treated at the same time daily. Routine syndrome differentiation group with reference to "Experimental Acupuncture and moxibustion" selected points, select Shuigou, Hegu and Sanyinjiao with the method of flat tonifying and reducing, once a day, with 30 min. for each time. According to Zheng Kuishan's Meridian flow and Ling Gui's eight methods, according to Xu's Meridian fluid injection, Na-Jia group was treated with acupuncture at acupoints in accordance with the date, and the manipulation and the time of needle retention were the same as the syndrome differentiation group. The pathological changes of neurons were observed and the concentration of neuron specific enolase (NSE), nerve growth factor (NGF),) basic fibroblast growth factor (b FGF) in the brain homogenate was detected by enzyme linked immunosorbent assay (ELISA). Results: compared with the normal group, the neuronal structure of the MCAO group was damaged, and the dynamic expression of NSE,NGF,b FGF in the brain of the MCAO rats was increased first and then decreased. All the treatment groups could inhibit the increase of NSE and increase the NGF,. The effect of the content of b FGF on the dynamic expression increased at first and then decreased. The effect of the combined group was better than that of the routine group in the early stage. Conclusion: acupuncture can promote the structural repair of hippocampal CA1 neurons in MCAO rats, promote the expression of NGF,b FGF in ischemic brain homogenate of MCAO rats, and inhibit the expression of NSE. The effect of NAA group and combined group is better than that of routine acupuncture. Acupuncture promoted the activation, proliferation and differentiation of neural stem cells after cerebral ischemia at the early stage of ischemia, suggesting that the early intervention of acupuncture was more obvious.
【作者单位】：甘肃中医药大学;甘肃省人民医院;
【基金】：甘肃省财政厅基本科研业务费专项资金项目(2010-6)
【分类号】：R-332;R245

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