艾灸肺俞、膏肓俞治疗肺纤维化大鼠对E-cad、α-SMA、vimentin的影响

发布时间：2018-12-08 14:38

【摘要】：目的:动态观察艾灸肺俞、膏肓俞治疗肺纤维化大鼠E-钙黏蛋白(E-cad)、α-平滑肌蛋白(α-SMA)、波形蛋白(vimentin)几种上皮-间质转分化(EMT)标志物的表达。方法:选用SD雄性大鼠44只,随机分为4组:正常对照组、模型组、艾灸治疗组、泼尼松治疗组。除正常对照组外,其余三组均采用博来霉素灌入气管复制肺纤维化模型,治疗组从造模后第1天开始分别艾灸及泼尼松灌胃治疗,在造模后第11天、第23天、第35天取材。采用HE染色、masson染色观察肺组织病理变化和采用免疫组化法检测E-cad、α-SMA、vimentin的表达。结果:艾灸组、泼尼松组与同时段模型组相比,肺组织病理程度明显降低,上皮细胞标记物E-cad表达升高,间质细胞标记物α-SMA、vimentin表达降低。结论:艾灸治疗能升高E-cad水平,降低α-SMA、vimentin水平,降低肺泡炎程度及延缓肺纤维化进程。
[Abstract]:Aim: to observe the expression of E-cadherin (E-cad) and 伪 -smooth muscle protein (伪 SMA), vimentin (vimentin) in the treatment of pulmonary fibrosis rats with moxibustion and ointment. Methods: 44 male SD rats were randomly divided into 4 groups: normal control group, model group, moxibustion group and prednisone group. Except the normal control group, the other three groups were treated with bleomycin into the trachea to make pulmonary fibrosis model. The treatment group was treated with moxibustion and prednisone respectively on the 1st day after the model was made. The materials were obtained on the 11th, 23rd and 35th day after the model was made. HE staining and masson staining were used to observe the pathological changes of lung tissue and the expression of E-cad- 伪-SMA,vimentin was detected by immunohistochemical method. Results: in moxibustion group and prednisone group, compared with the model group, the pathological degree of lung tissue was significantly decreased, the expression of E-cad and 伪-SMA,vimentin, the marker of epithelial cells were increased, and the expression of 伪-SMA,vimentin in interstitial cells was decreased significantly in the moxibustion group and prednisone group. Conclusion: moxibustion treatment can increase the level of E-cad, decrease the level of 伪-SMA,vimentin, decrease the degree of alveolitis and delay the progress of pulmonary fibrosis.
【作者单位】：成都中医药大学;
【基金】：国家自然科学基金(81373734)
【分类号】：R245

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