电针对高血压前期Dahl盐敏感大鼠肾脏保护作用机制研究

发布时间：2018-12-25 12:26

【摘要】：目的:观察电针"曲池""足三里"穴对高血压前期Dahl盐敏感(DS)大鼠肾脏NO/c GMP/PKG信号通路的影响及组织形态的改变,探讨电针"曲池""足三里"对高血压前期肾脏损害保护作用机制。方法:盐抵抗大鼠(DR)10只作为正常组;DS大鼠30只,随机分为模型组、针刺组和非穴位组,每组10只。采用高盐饲料喂养的方法诱发高血压前期模型。电针组电针"曲池""足三里"穴,1次/d,6次/周,共5周。非穴位组取双侧髂嵴上10~15 mm、后正中线旁开20mm区段内一个固定对照点,治疗方法同电针组。测量大鼠治疗期间血压变化情况以及治疗后血清血清尿素氮(UREA)、血肌酐(CREA)、血尿酸(UA)含量;ELISA法检测NO含量;荧光定量PCR检测大鼠肾组织溶解型鸟苷酸环化酶(s GC)、蛋白激酶G(PKG)的mRNA表达;HE染色检测大鼠肾组织病理形态变化。结果:治疗期间,电针组大鼠血压逐步下降。与正常组相比,模型组血清UREA、CREA、UA含量显著升高(P0.05),NO含量显著降低(P0.05),s GC、PKG mRNA表达显著降低(P0.05);与模型组相比,电针组血清UREA、CREA、UA含量显著降低(P0.05),NO含量显著升高(P0.05),s GC、PKG mRNA表达显著升高(P0.05)。结论:电针能有效降低高血压前期DS大鼠血压,提高NO含量、c GMP、PKG基因表达,改善组织小管扩张、间质炎症细胞浸润等,这可能是电针通过NO/c GMP/PKG信号通路发挥对高血压前期DS大鼠肾脏保护作用机制之一。
[Abstract]:Objective: to observe the effect of electroacupuncture at "qu Chi" and "Zusanli" on the renal NO/c GMP/PKG signaling pathway and the changes of tissue morphology in pre-hypertensive Dahl salt-sensitive (DS) rats. To explore the protective mechanism of electroacupuncture (EA) on renal damage in prehypertensive stage of Zusanli (Zusanli). Methods: ten (DR) rats with salt resistance and 30 DS rats were randomly divided into three groups: model group, acupuncture group and non-acupoint group with 10 rats in each group. The prehypertension model was induced by high salt feed. In the electroacupuncture group, the acupoints of Zusanli, once a day, 6 times a week for 5 weeks. After 1015 mm, of bilateral iliac crest, a fixed control point in 20mm segment was opened in the non-acupoint group. The treatment method was the same as that in the electroacupuncture group. Serum urea nitrogen (UREA), serum creatinine (CREA), serum uric acid (UA), serum urea nitrogen (UREA), serum creatinine (CREA), serum uric acid (UA) were measured in rats during treatment, and NO content was detected by ELISA. Fluorescence quantitative PCR was used to detect the mRNA expression of (s GC), protein kinase G (PKG) in rat renal tissue, and HE staining was used to detect the histopathological changes of renal tissue. Results: during the treatment period, the blood pressure of the rats in the electroacupuncture group decreased gradually. Compared with the normal group, the serum UREA,CREA,UA content in the model group was significantly increased (P0.05), NO content significantly decreased (P0.05), s GC,PKG mRNA expression significantly decreased (P0.05); Compared with the model group, the serum UREA,CREA,UA content in the electroacupuncture group was significantly lower (P0.05), NO content was significantly higher (P0.05), s GC,PKG mRNA expression was significantly higher (P0.05). Conclusion: electroacupuncture can effectively reduce blood pressure, increase NO content, c GMP,PKG gene expression, improve tubule dilatation and interstitial inflammatory cell infiltration in prehypertensive DS rats. This may be one of the mechanisms of renal protection by electroacupuncture through NO/c GMP/PKG signaling pathway in prehypertensive DS rats.
【作者单位】：辽宁中医药大学中医脏象理论及应用教育部重点实验室;
【基金】：国家重点基础研究发展计划(973计划)课题(2012CB518503) 辽宁省教育厅创新团队课题(LT2014021)
【分类号】：R245.97

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