巨刺对脑缺血再灌注损伤大鼠脑组织VEGF和Ang-1表达的影响

发布时间：2019-01-14 14:27

【摘要】：目的:观察巨刺对MCAO/R模型大鼠大脑皮质缺血区VEGF和Ang-1表达水平的影响,探讨巨刺治疗急性脑缺血再灌注损伤大鼠的作用机制。方法:将100只雄性SD大鼠随机分为空白组、假手术组、模型组、巨刺组(即健侧针刺组)、患侧针刺组,各20只,各组再分别分为3d亚组和14d亚组,每组各10只。采用Zea Longa线栓法制备MCAO/R模型。2小时后再灌注。24h后分别进行治疗。巨刺组针刺“人中”、“百会”及电针治疗健侧肢体的曲池、内关、足三里、三阴交;患侧针刺组针刺“人中”、“百会”及电针治疗患侧肢体的曲池、内关、足三里、三阴交。造模后和疗程结束后予神经功能评分,观察大鼠缺血脑组织结构;采用免疫组化法(S-ABC法)测定缺血区大脑皮质VEGF和Ang-1表达情况。结果:1.3d和14d亚组中,造模后各组均出现不同程度神经功能缺损症状,提示模型制备成功.与模型组相比,巨刺组、患侧针刺组神经功能均无统计学差异(P0.05)。治疗3天后,巨刺组、患侧针刺组分别与模型组比较,神经功能均得到改善,而巨刺组、患侧针刺组比较无统计学差异。(P0.05)巨刺组、患侧针刺组治疗前后各自比较差异均有意义(P0.05)。治疗14天后,巨刺组、患侧针刺组与模型组比较神经功能得到改善(P0.05),巨刺组较患侧针刺组神经功能改善较明显(P0.05)。巨刺、患侧针刺组治疗14天后与治疗前比较,神经功能改善均具有统计学意义(P0.01)。2.与模型组相比,针刺治疗后大鼠缺血区脑组织损伤程度均明显改善,其中巨刺组较患侧针刺组改善明显,且14d亚组较3d亚组缺血脑组织改善明显。3.巨刺组、患侧针刺组VEGF和Ang-1的表达均增多(P0.05);而巨刺组VEGF和Ang-1的表达均较患侧针刺组增多明显(P0.05)。结论:巨刺具有改善急性缺血再灌注损伤大鼠缺血区脑组织损伤程度,改善脑缺血大鼠神经功能缺损,其机制可能与显著上调脑组织VEGF和Ang-1表达水平有关。
[Abstract]:Aim: to observe the effects of giant prickles on the expression of VEGF and Ang-1 in cerebral cortex ischemia area of MCAO/R model rats and to explore the mechanism of giant prickles in the treatment of acute cerebral ischemia-reperfusion injury in rats. Methods: one hundred male SD rats were randomly divided into three groups: blank group, sham operation group, model group, giant prick group (healthy side acupuncture group) and affected side acupuncture group (20 rats). Each group was subdivided into 3 d subgroup and 14 d subgroup with 10 rats in each group. The model of MCAO/R was established by Zea Longa thread embolization method. 2 hours after reperfusion, 24 hours after treatment, respectively. Giant stab group acupuncture "human middle", "Baihui" and electroacupuncture treatment of the healthy side of the limb qu Chi, Neiguan, Zusanli, Sanyinjiao; The affected side acupuncture group acupuncture "human middle", "Baihui" and electroacupuncture treatment of the affected side limb qu Chi, Neiguan, Zusanli, Sanyinjiao. After the model was made and the course of treatment was finished, the neural function score was used to observe the structure of the ischemic brain, and the expression of VEGF and Ang-1 in the cerebral cortex of the ischemic area was determined by immunohistochemical method (S-ABC). Results: 1. In the subgroup of day 3 and day 14, the symptoms of different degrees of nerve function defect appeared in each group after the model was made, which indicated that the model was successfully prepared. Compared with the model group, there was no significant difference in nerve function between the giant prick group and the affected side acupuncture group (P0.05). After 3 days of treatment, the nerve function of the giant prick group and the affected side acupuncture group were all improved compared with the model group, but there was no statistical difference between the giant prick group and the affected side acupuncture group. (P0.05) the nerve function of the giant needling group was significantly higher than that of the model group. There were significant differences between the two groups before and after treatment (P0.05). After 14 days of treatment, the nerve function of the giant prick group and the affected side acupuncture group was improved compared with the model group (P0.05), and the nerve function of the giant prick group was significantly improved than that of the affected side acupuncture group (P0.05). After 14 days of treatment, the improvement of nerve function was statistically significant (P0.01). Compared with the model group, the degree of cerebral tissue injury in ischemic area of rats after acupuncture treatment was significantly improved, in which the giant prick group was significantly improved than the affected side acupuncture group, and the 14 d subgroup was significantly improved than that of 3 d subgroup. The expression of VEGF and Ang-1 in the giant prick group was higher than that in the affected side acupuncture group (P0.05), while the expression of VEGF and Ang-1 in the giant prick group was significantly higher than that in the affected side acupuncture group (P0.05). Conclusion: Giant prickly can improve the degree of cerebral tissue injury in rats with acute ischemia-reperfusion injury and improve the neural function defect in ischemic rats. The mechanism may be related to the up-regulation of the expression of VEGF and Ang-1 in the brain tissue of rats with acute ischemia-reperfusion injury.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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