大承气汤治疗重症急性胰腺炎肠功能障碍的机制研究

发布时间：2019-01-20 16:12

【摘要】：目的:探讨大承气汤治疗大鼠重症急性胰腺炎(SAP)肠功能障碍的可能机制。方法:将54只雄性SD大鼠随机分为假手术组(SO组)、重症急性胰腺炎肠功能障碍组(SAP组)和大承气汤治疗组,每组18只。经胆胰管内加压注射3.5%牛磺胆酸钠0.1 m L/100 g体质量制备SAP肠功能障碍模型,治疗组在造模前给予10%的大承气汤剂(9.18 g/kg体质量)灌胃1次,SAP组和SO组则灌注等量0.9%氯化钠溶液。每组于造模后3、6、12 h各处死6只大鼠并立即心脏取血,检测血清淀粉酶、脂肪酶水平,酶联免疫吸附试验检测血清5-羟色胺(5-HT)、白介素6(IL-6)含量。同时检测6h时间点各组大鼠距屈氏韧带10 cm空肠、末端回肠及乙状结肠组织中5-HT7R的表达情况。结果:造模后SAP组和治疗组血清淀粉酶、脂肪酶及5-HT水平均明显高于SO组(P0.05),且每个时间点治疗组均较SAP组显著降低(P0.05)。与SO组相比,SAP组5-HT7R蛋白及阳性细胞面积表达水平在空肠、末端回肠及乙状结肠均明显增高(P0.05);与SAP组比较,治疗组各部位5-HT7R蛋白及阳性细胞面积表达均减少(P0.05)。结论:血清5-HT的增加过度激活5-HT7R可能是SAP发生肠功能障碍的原因之一,而大承气汤可能是通过减少5-HT7R的表达来促进肠功能恢复。
[Abstract]:Objective: to explore the possible mechanism of Dachengqi decoction in the treatment of (SAP) intestinal dysfunction in rats with severe acute pancreatitis. Methods: Fifty-four male SD rats were randomly divided into sham-operated group (SO group), severe acute pancreatitis group (SAP group) and Dachengqi decoction group (18 rats in each group). The model of intestinal dysfunction of SAP was established by injecting 3.5% sodium taurocholate 0.1 mL / 100g body mass into the biliary and pancreatic duct. The treatment group was given 10% Dachengqi decoction (9.18 g/kg body mass) once a time before making the model. SAP group and SO group were perfused with 0.9% sodium chloride solution. In each group, 6 rats were killed in each group for 12 hours, and blood was taken from the heart. Serum amylase and lipase levels were measured. Serum 5-hydroxytryptamine (5-HT) and interleukin-6 (IL-6) levels were measured by enzyme-linked immunosorbent assay (Elisa). At the same time, the expression of 5-HT7R in jejunum, terminal ileum and sigmoid colon was detected at 6 h. Results: the levels of serum amylase, lipase and 5-HT in SAP group and treatment group were significantly higher than those in SO group (P0.05), and the levels of serum amylase, lipase and 5-HT in treatment group were significantly lower than those in SAP group at each time point (P0.05). Compared with SO group, the expression of 5-HT7R protein and positive cell area in SAP group were significantly higher in jejunum, terminal ileum and sigmoid colon (P0.05). Compared with SAP group, 5-HT7R protein and positive cell area in treatment group were decreased (P0.05). Conclusion: the increase of serum 5-HT and excessive activation of 5-HT7R may be one of the causes of intestinal dysfunction in SAP, while Dachengqi decoction may promote the recovery of intestinal function by reducing the expression of 5-HT7R.
【作者单位】：无锡市第三人民医院;
【基金】：国家自然科学基金项目(81200161)
【分类号】：R259

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