电针内关穴对心肌缺血小鼠心肌ATP敏感钾通道蛋白及蛋白激酶的影响

发布时间：2019-01-21 10:22

【摘要】：目的:观察电针内关穴对急性心肌缺血ASIC3-/-小鼠ATP敏感性钾离子通道亚基(S UR2A、SUR2B、Kir6.1、Kir6.2)和蛋白激酶(PKA、PKC-β)表达的影响。材料与方法:选取健康雄性8周龄ASIC3-/-小鼠(C57BL/6)40只,由北京维通利华实验动物技术有限公司提供。适应性喂养一周后开始实验。将小鼠随机分为6组,每组6只,包括:空白组、模型组、内关组、列缺组、足三里组和非经非穴组。心肌缺血模型采用皮下多点注射异丙肾上腺素(20mg/kg)制备,连续两天,空白组不作处理。造模前后分别测量各组小鼠的心电图。分组进行电针干预,干预结束后再次测量各组小鼠的心电图、心室肌组织ATP敏感性钾离子通道蛋白及蛋白激酶的表达量。结果:1.针刺干预后内关组、列缺组和足三里组的T波振幅相比模型组都显著回升,非经非穴组变化无统计意义,内关组的T波振幅与列缺组、足三里组的T波振幅没有统计差别。2.针刺干预后,与模型组相比,内关组、列缺组和足三里组的ATP敏感钾通道蛋白(S UR2A、SUR2B、Kir6.1、Kir6.2)表达都明显下降。3.针刺干预后,与模型组比较,内关组和列缺组的PKA、PKC-β含量均明显下降。结论:1.电针内关穴可以改善心肌缺血小鼠的心电图T波改变。2.电针内关穴可以使心肌缺血小鼠大量表达的ATP敏感性钾离子通道蛋白SUR2A、SU R2B、Kir6.1及Kir6.2亚基表达减少。3.电针内关穴能使心肌缺血小鼠大量表达的蛋白激酶(PKA和PKC-β)表达减少。4.对于心肌缺血小鼠模型,电针内关穴的效果优于列缺穴和足三里穴,对Kir6.2、SUR2A亚基和PKA的调节体现了内关穴的循经特异性。
[Abstract]:Aim: to observe the effect of electroacupuncture at Neiguan point on the expression of ATP sensitive potassium channel subunit (S UR2A,SUR2B,Kir6.1,Kir6.2) and protein kinase (PKA,PKC- 尾) in ASIC3-/- mice with acute myocardial ischemia. Materials and methods: forty healthy 8-week-old ASIC3-/- mice (C57BL/6) were selected and supplied by Beijing Weitonglihua Experimental Animal Technology Co., Ltd. The experiment began after a week of adaptive feeding. The mice were randomly divided into 6 groups, including blank group, model group, Neiguan group, lack group, Zusanli group and non-meridian non-acupoint group. Myocardial ischemia model was prepared by subcutaneous injection of isoproterenol (20mg/kg) for two days without treatment. Electrocardiogram (ECG) of each group of mice was measured before and after modeling. After the intervention, electrocardiogram (ECG) and the expression of ATP sensitive potassium channel protein and protein kinase in ventricular muscle tissue were measured again. Results: 1. After acupuncture intervention, T wave amplitudes in Neiguan group, Zusanli group and Zusanli group increased significantly compared with model group, but there was no statistical significance in non-meridian non-acupoint group. There was no statistical difference in T wave amplitude between Neiguan group and train deficiency group and Zusanli group. 2. Compared with model group, the expression of ATP sensitive potassium channel protein (S UR2A,SUR2B,Kir6.1,Kir6.2) in Neiguan group, column deficiency group and Zusanli group was significantly decreased after acupuncture intervention. Compared with the model group, the content of PKA,PKC- 尾 in the Neiguan group and the column deficiency group decreased significantly after acupuncture intervention. Conclusion: 1. Electroacupuncture at Neiguan point can improve the changes of T wave in myocardial ischemia mice. 2. Electroacupuncture at Neiguan point could decrease the expression of ATP sensitive K + channel protein SUR2A,SU R2BX Kir6.1 and Kir6.2 subunit in myocardial ischemia mice. Electroacupuncture at Neiguan point reduced the expression of protein kinase (PKA) and protein kinase 尾 (PKC- 尾) in mice with myocardial ischemia. For the model of myocardial ischemia, the effect of electroacupuncture at Neiguan acupoint was better than that of Lie-deficiency point and Zusanli point, and the regulation of Kir6.2,SUR2A subunit and PKA reflected the specificity of Neiguan acupoint along meridian.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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