针刺对结肠吻合术后大鼠结肠Cajal间质细胞受体酪氨酸激酶c-kit表达和结肠组织一氧化碳及血红素氧合酶的影响

发布时间：2019-02-14 07:08

【摘要】：目的:观察针刺对结肠吻合术后结肠Cajal间质细胞(ICCs)受体酪氨酸激酶c-kit表达和结肠组织一氧化碳(CO)及血红素氧合酶(HO)的影响,探讨针刺改善胃肠动力的机制。方法:SD大鼠随机分为空白组、模型组和针刺组,采用结肠吻合术造模,每组分术后3、5、10d3个时段,共9个亚组,每亚组10只。针刺组于术后次日予针刺干预,取双侧"足三里""三阴交""太冲"穴,留针15min,1次/d。记录术后首次排便时间,计算各时段小肠推进率,采用免疫组化法检测结肠c-kit表达,用生化法检测结肠组织CO含量,ELISA法检测结肠组织HO-1、HO-2活性。结果:与空白组比较,模型组小肠推进率下降(P0.05);c-kit表达水平减少(P0.05);术后3、5d结肠组织CO含量和HO-1活性升高(P0.05),后恢复正常;术后3d结肠组织HO-2活性升高(P0.05),而术后5、10d HO-2活性降至低于空白组(P0.05)。与模型组比较,针刺组术后首次排便时间缩短(P0.05);小肠推进率升高(P0.05);c-kit表达水平增加(P0.05);术后3、5d结肠组织CO含量和HO-1活性降低(P0.05);术后3d结肠组织HO-2活性降低(P0.05),而术后10dHO-2活性升高(P0.05)。与术后3d比较,模型组及针刺组术后5d亚组小肠推进率升高,c-kit表达水平增加,结肠组织CO含量和HO-1、HO-2活性均降低(均P0.05);与术后5d比较,模型组术后10d亚组小肠推进率升高,c-kit表达水平增加,结肠组织CO含量和HO-1活性均降低(均P0.05)。结论:针刺能降低结肠组织CO含量和HO-1、HO-2活性,升高c-kit表达水平,促进术后ICCs修复,进而改善术后胃肠动力。
[Abstract]:Aim: to observe the effects of acupuncture on the expression of (ICCs) receptor tyrosine kinase (c-kit) in interstitial cells of Cajal after colonic anastomosis and the changes of carbon monoxide (CO) and heme oxygenase (HO) in colon tissue, and to explore the mechanism of acupuncture in improving gastrointestinal motility. Methods: SD rats were randomly divided into three groups: blank group, model group and acupuncture group. Colonic anastomosis was performed with 10 rats in 9 subgroups. The acupuncture group was treated with acupuncture intervention the next day after operation. The bilateral points of "Zusanli", "Sanyinjiao" and "Taochong" were taken, and the needle was kept for 15 minutes once a day. The first defecation time after operation was recorded, and the intestinal propulsion rate was calculated. The expression of c-kit in colon was detected by immunohistochemical method, the content of CO in colon tissue was detected by biochemical method, and the activity of HO-1,HO-2 in colon tissue was detected by ELISA method. Results: compared with the blank group, the intestinal propulsion rate of the model group decreased (P0.05), the expression of c-kit decreased (P0.05), the content of CO and the activity of HO-1 in colon tissue increased 3 days after operation (P0.05), and then returned to normal. The activity of HO-2 in colon tissue increased 3 days after operation (P0.05), but the activity of HO-2 decreased to lower than that of blank group on the 10th day after operation (P0.05). Compared with the model group, the first defecation time in the acupuncture group was shortened (P0.05), the intestinal propulsion rate was increased (P0.05), the expression of c-kit was increased (P0.05). The content of CO and the activity of HO-1 in colon tissue decreased 3 days after operation (P0.05), the activity of HO-2 in colon tissue decreased 3 days after operation (P0.05), but the activity of 10dHO-2 increased after operation (P0.05). Compared with the 3rd day after operation, the intestinal propulsion rate, c-kit expression, CO content and HO-1,HO-2 activity of colon tissue in model group and acupuncture group increased on the 5th day after operation (P0.05). Compared with 5 days after operation, the small intestinal propulsion rate and c-kit expression increased, and the content of CO and HO-1 activity in colon tissue decreased in the model group 10 days after operation (P0.05). Conclusion: acupuncture can decrease the CO content and HO-1,HO-2 activity, increase the expression of c-kit, promote the repair of ICCs after operation, and then improve the gastrointestinal motility after operation.
【作者单位】：广州市第八人民医院中医科;广州中医药大学针灸推拿学院;
【基金】：国家自然科学基金(No.81202756) 广东省自然科学基金(No.S 2012040007674)
【分类号】：R245

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