“秩边透水道”针法治疗大鼠原发性痛经作用机制的实验研究
发布时间：2019-02-15 03:02
【摘要】：目的通过建立大鼠原发性痛经模型,观察“秩边透水道”针法对痛经大鼠一般行为,扭体次数,血清白介素-1(IL-1)、白介素-6(IL-6)、肿瘤坏死因子-a(TNF-a),血浆β-内啡肽(β-EP),下丘脑组织5-羟色胺(5-HT)等多个指标并加以分析,探讨“秩边透水道”针法治疗原发性痛经的作用机制,为临床新技术的出现提供准确的实验依据,以便更好地推广临床。方法选用健康雌性SD大鼠40只(230g±15g),造模前实验室常规饲养一周使其适应环境,采用随机数字表法将动物分为4组:空白组、模型组、针刺组、秩边组,每组10只。实验采用催产素致大鼠痛经模型:模型组、针刺组及秩边组大鼠股部皮下注射苯甲酸雌二醇连续10天,第1、10天0.5mg/只,第2—9天0.2mg/只,末次给药1 h后,腹腔注射缩宫素2u/只;空白组大鼠每日股部皮下注射同等剂量的生理盐水。从造模开始第一天即进行干预,连续5天,每次20min,空白组、模型组仅抓取固定,针刺组每天造模后针刺关元、双侧三阴交穴位,秩边组于每天造模后即进行“秩边透水道”针法干预;在造模和治疗过程中观察大鼠的毛色、饮食、行动、体重等一般状况,记录腹腔注射缩宫素大鼠30分钟内扭体次数,采用放射免疫法检测各组大鼠血清IL-1、IL-6、TNF-a、血浆β-EP、下丘脑组织5-HT的含量,统计分析相关指标,汇总结果。结果1、空白组大鼠一般状况良好,未见明显异常。模型组大鼠毛色暗淡发黄,特别是腹部有明显的发黄潮湿,食欲降低,动作迟缓,体重下降。针刺组与秩边组大鼠较模型组有很大改善。2、空白组大鼠没有出现扭体反应,模型组、针刺组及秩边组大鼠全部发生扭体反应,模型组大鼠扭体反应较明显,大鼠腹部收缩内凹,躯干与后肢伸展,臀部与一侧肢体内旋。3、模型组大鼠血浆β-EP含量高于空白组,秩边组及针刺组的含量均高于模型组,而秩边组又高于针刺组,均差异显著(P0.05);模型组与空白组比较,大鼠下丘脑组织中5-HT含量明显降低,有显着差异(P0.05);针刺组、秩边组与模型组比较,大鼠下丘脑组织5-HT含量存在明显差异(P0.05),秩边组与针刺组之间没有明显差异;模型组与空白组比较,大鼠血清TNF-a、IL-1、IL-6含量明显升高,有统计学意义(P0.05);针刺组、秩边组与模型组比较,大鼠血清TNF-a、IL-1、IL-6含量存在明显差异(P0.05),秩边组与针刺组之间没有明显差异。结论原发性痛经大鼠实验模型制备成功;“秩边透水道”针法能明显改善原发性痛经大鼠模型的一般状况,降低扭体次数,提高大鼠下丘脑组织5-HT及血浆β-EP的含量,降低血清IL-1、IL-6、TNF-a的含量,初步阐明了其治疗原发性痛经的作用机制,有利于基础研究与临床应用的衔接,为新技术的出现提供实验支持。
[Abstract]:Objective to observe the general behavior, writhing times, serum interleukin-1 (IL-1) and interleukin-6 (IL-6) in rats with dysmenorrhea by establishing a rat model of primary dysmenorrhea. Tumor necrosis factor-a (TNF-a), plasma 尾 -endorphin (尾 -endorphin) and 5-hydroxytryptamine (5-HT) in hypothalamus were analyzed. To provide accurate experimental basis for the emergence of new clinical technology, in order to better promote clinical. Methods Forty healthy female SD rats (230g 卤15g) were randomly divided into four groups: blank group, model group, acupuncture group and rank side group. The model of dysmenorrhea induced by oxytocin: the rats in the model group, acupuncture group and rank side group were injected estradiol benzoate subcutaneously into the femoris for 10 days, only 0.5mg/ on the 1st day, 0.2mg/ on the 2nd to 9th day, and 1 hour after the last administration of estradiol benzoate. Intraperitoneal injection of oxytocin 2u/; Rats in the blank group were subcutaneously injected with the same dose of normal saline daily. Intervention was carried out from the first day of modeling for 5 days each time for 20 minutes. The blank group and the model group only grasped fixation. The acupuncture group needled Guanyuan after modeling every day, and the acupoints of Sanyinjiao on both sides were punctured. In the rank side group, the needle intervention of "rank edge permeating waterway" was carried out immediately after making model every day. In the course of modeling and treatment, we observed the general condition of color, diet, exercise and body weight of rats, recorded the times of writhing in 30 minutes after intraperitoneal injection of oxytocin, and detected the serum IL-1,IL-6, of rats by radioimmunoassay. The content of 5-HT in hypothalamus tissue of plasma 尾-EP, in TNF-a, was analyzed statistically and the results were summarized. Results 1. The normal condition of the blank group was good and no obvious abnormality was found. In the model group, the hair color was dim and yellowish, especially in the abdomen with obvious yellowish humidity, decreased appetite, slow movement and weight loss. Compared with the model group, the acupuncture group and the rank side group showed significant improvement. 2. There was no writhing reaction in the blank group, all the rats in the model group, the acupuncture group and the rank side group had writhing reaction, and the writhing reaction was more obvious in the model group. The contents of plasma 尾-EP in the model group were higher than those in the blank group, the contents of 尾-EP in the rank side group and acupuncture group were higher than those in the model group, and the rank side group was higher than that in the acupuncture group, and the content of 尾-EP in the rank side group was higher than that in the acupuncture group. The difference was significant (P0.05). Compared with the blank group the content of 5-HT in the hypothalamus of the model group was significantly lower than that of the blank group (P0.05). In acupuncture group, rank side group and model group, the content of 5-HT in hypothalamus was significantly different (P0.05), but there was no significant difference between rank side group and acupuncture group. Compared with the blank group, the serum TNF-a,IL-1,IL-6 content in the model group was significantly higher than that in the blank group (P0.05). Compared with the model group, the serum TNF-a,IL-1,IL-6 content in acupuncture group, rank edge group and model group was significantly different (P0.05), but there was no significant difference between the rank side group and acupuncture group. Conclusion the experimental model of primary dysmenorrhea in rats is successful. The acupuncture method of "rank side through waterway" could obviously improve the general condition of primary dysmenorrhea rat model, decrease the number of writhing, increase the content of 5-HT and plasma 尾-EP in hypothalamus, and decrease the content of serum IL-1,IL-6,TNF-a. The mechanism of treatment of primary dysmenorrhea is elucidated preliminarily, which is beneficial to the connection between basic research and clinical application, and provides experimental support for the emergence of new technology.
【学位授予单位】：山西中医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
本文编号：2422893
[Abstract]:Objective to observe the general behavior, writhing times, serum interleukin-1 (IL-1) and interleukin-6 (IL-6) in rats with dysmenorrhea by establishing a rat model of primary dysmenorrhea. Tumor necrosis factor-a (TNF-a), plasma 尾 -endorphin (尾 -endorphin) and 5-hydroxytryptamine (5-HT) in hypothalamus were analyzed. To provide accurate experimental basis for the emergence of new clinical technology, in order to better promote clinical. Methods Forty healthy female SD rats (230g 卤15g) were randomly divided into four groups: blank group, model group, acupuncture group and rank side group. The model of dysmenorrhea induced by oxytocin: the rats in the model group, acupuncture group and rank side group were injected estradiol benzoate subcutaneously into the femoris for 10 days, only 0.5mg/ on the 1st day, 0.2mg/ on the 2nd to 9th day, and 1 hour after the last administration of estradiol benzoate. Intraperitoneal injection of oxytocin 2u/; Rats in the blank group were subcutaneously injected with the same dose of normal saline daily. Intervention was carried out from the first day of modeling for 5 days each time for 20 minutes. The blank group and the model group only grasped fixation. The acupuncture group needled Guanyuan after modeling every day, and the acupoints of Sanyinjiao on both sides were punctured. In the rank side group, the needle intervention of "rank edge permeating waterway" was carried out immediately after making model every day. In the course of modeling and treatment, we observed the general condition of color, diet, exercise and body weight of rats, recorded the times of writhing in 30 minutes after intraperitoneal injection of oxytocin, and detected the serum IL-1,IL-6, of rats by radioimmunoassay. The content of 5-HT in hypothalamus tissue of plasma 尾-EP, in TNF-a, was analyzed statistically and the results were summarized. Results 1. The normal condition of the blank group was good and no obvious abnormality was found. In the model group, the hair color was dim and yellowish, especially in the abdomen with obvious yellowish humidity, decreased appetite, slow movement and weight loss. Compared with the model group, the acupuncture group and the rank side group showed significant improvement. 2. There was no writhing reaction in the blank group, all the rats in the model group, the acupuncture group and the rank side group had writhing reaction, and the writhing reaction was more obvious in the model group. The contents of plasma 尾-EP in the model group were higher than those in the blank group, the contents of 尾-EP in the rank side group and acupuncture group were higher than those in the model group, and the rank side group was higher than that in the acupuncture group, and the content of 尾-EP in the rank side group was higher than that in the acupuncture group. The difference was significant (P0.05). Compared with the blank group the content of 5-HT in the hypothalamus of the model group was significantly lower than that of the blank group (P0.05). In acupuncture group, rank side group and model group, the content of 5-HT in hypothalamus was significantly different (P0.05), but there was no significant difference between rank side group and acupuncture group. Compared with the blank group, the serum TNF-a,IL-1,IL-6 content in the model group was significantly higher than that in the blank group (P0.05). Compared with the model group, the serum TNF-a,IL-1,IL-6 content in acupuncture group, rank edge group and model group was significantly different (P0.05), but there was no significant difference between the rank side group and acupuncture group. Conclusion the experimental model of primary dysmenorrhea in rats is successful. The acupuncture method of "rank side through waterway" could obviously improve the general condition of primary dysmenorrhea rat model, decrease the number of writhing, increase the content of 5-HT and plasma 尾-EP in hypothalamus, and decrease the content of serum IL-1,IL-6,TNF-a. The mechanism of treatment of primary dysmenorrhea is elucidated preliminarily, which is beneficial to the connection between basic research and clinical application, and provides experimental support for the emergence of new technology.
【学位授予单位】：山西中医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
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