回药核心方油溶液对离体大鼠胸主动脉血管环的舒张作用及机制研究
发布时间：2019-03-07 08:58
【摘要】：目的:研究回药核心方油溶液对离体大鼠胸主动脉血管环的舒张作用及机制,为其用于心血管疾病的治疗提供参考。方法:取出大鼠胸主动脉血管环后浸泡于克氏营养液(K-H)中,以1×10~(-6)mol/L去甲肾上腺素(PE)或60 mmol/L氯化钾(KCl)致血管环收缩,采用生物信号采集分析系统测定0.020 4、0.040 8、0.061 2、0.081 6、0.102 0 mg/mL核心方油溶液对血管环的舒张作用,计算舒张率;分别以0.1 mmol/L一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME)、环氧合酶抑制剂吲哚美辛(INDO)、钾离子通道阻滞剂格列本脲(Gli)孵育血管环20 min后,测定上述5种质量浓度核心方油溶液对PE预收缩血管环的舒张作用,计算舒张率;试验均以K-H溶液为空白对照。结果:与空白对照比较,0.020 4~0.102 0 mg/mL核心方油溶液对PE、KCl预收缩血管环均有明显的舒张作用(P0.05或P0.01),且具有浓度依赖性。INDO预处理后可减弱核心方油溶液对PE预收缩血管环的舒张作用,舒张率较空白对照组差异无统计学意义(P0.05);而Gli、L-NAME预处理不影响核心方油溶液对PE预收缩血管环的舒张作用,舒张率较空白对照组仍明显升高(P0.05或P0.01)。结论:核心方油溶液可呈浓度依赖性地舒张PE、KCl预收缩的胸主动脉血管环,其作用机制可能与激活环氧合酶途径有关。
[Abstract]:Aim: to study the vasodilation of isolated rat thoracic aortic rings and its mechanism in order to provide reference for the treatment of cardiovascular diseases. Methods: rat thoracic aortic rings were taken out and immersed in Kjh solution. The rings were induced by 1 脳 10 ~ (- 6) mol/L norepinephrine (PE) or 60 mmol/L potassium chloride (KCl) (KCl _ 2), and the vasoconstriction was induced by 1 脳 10 ~ (- 6) mmol/L noradrenaline (PE). The vasodilation effects of 0.020, 0.0408,0.061,200, 0.0816, 0.102 mg/mL core oil solution on vascular rings were measured by biological signal acquisition and analysis system, and the relaxation rate was calculated. The vascular rings were incubated with 0.1 mmol/L nitric oxide synthase inhibitor L-nitro arginine methyl ester (L-NAME) and indomethacin (INDO), potassium channel blocker glibenclamide (Gli) for 20 min, respectively. The vasodilation effect of the core oil solution of the above five concentrations on the pre-constrictive vascular rings of PE was measured and the relaxation rate was calculated. K H solution was used as the blank control. Results: compared with the blank control, the 0.020 mg/mL core oil solution had obvious vasodilation effect on the pre-constricted vascular rings of PE,KCl (P0.05 or P0.01), and there was no significant difference between the control group and the control group (P0.05 or P0.01). Indo pretreatment could attenuate the vasodilation effect of core oil solution on PE pre-contraction, and there was no significant difference in diastolic rate compared with the blank control group (P0.05). Pretreatment with Gli,L-NAME did not affect the vasodilation effect of the core oil solution on the pre-contraction of PE, and the diastolic rate was still significantly higher than that of the blank control group (P0.05 or P0.01). Conclusion: the core oil solution can dilate the thoracic aortic rings precontracted by PE,KCl in a concentration-dependent manner, the mechanism of which may be related to the activation of cyclooxygenase pathway.
【作者单位】： 宁夏医科大学总医院药剂科;宁夏医科大学药学院;宁夏回药现代化工程技术研究中心;宁夏回医药协同创新中心;宁夏医科大学回医药现代化省部共建教育部重点实验室;
【基金】：国家科技支撑计划课题(No.2013BAI11B07) 宁夏回族自治区科技攻关计划项目(No.2012) 大学生创新/创业计划项目(No.201510752008)
【分类号】：R29
，
本文编号：2435966
[Abstract]:Aim: to study the vasodilation of isolated rat thoracic aortic rings and its mechanism in order to provide reference for the treatment of cardiovascular diseases. Methods: rat thoracic aortic rings were taken out and immersed in Kjh solution. The rings were induced by 1 脳 10 ~ (- 6) mol/L norepinephrine (PE) or 60 mmol/L potassium chloride (KCl) (KCl _ 2), and the vasoconstriction was induced by 1 脳 10 ~ (- 6) mmol/L noradrenaline (PE). The vasodilation effects of 0.020, 0.0408,0.061,200, 0.0816, 0.102 mg/mL core oil solution on vascular rings were measured by biological signal acquisition and analysis system, and the relaxation rate was calculated. The vascular rings were incubated with 0.1 mmol/L nitric oxide synthase inhibitor L-nitro arginine methyl ester (L-NAME) and indomethacin (INDO), potassium channel blocker glibenclamide (Gli) for 20 min, respectively. The vasodilation effect of the core oil solution of the above five concentrations on the pre-constrictive vascular rings of PE was measured and the relaxation rate was calculated. K H solution was used as the blank control. Results: compared with the blank control, the 0.020 mg/mL core oil solution had obvious vasodilation effect on the pre-constricted vascular rings of PE,KCl (P0.05 or P0.01), and there was no significant difference between the control group and the control group (P0.05 or P0.01). Indo pretreatment could attenuate the vasodilation effect of core oil solution on PE pre-contraction, and there was no significant difference in diastolic rate compared with the blank control group (P0.05). Pretreatment with Gli,L-NAME did not affect the vasodilation effect of the core oil solution on the pre-contraction of PE, and the diastolic rate was still significantly higher than that of the blank control group (P0.05 or P0.01). Conclusion: the core oil solution can dilate the thoracic aortic rings precontracted by PE,KCl in a concentration-dependent manner, the mechanism of which may be related to the activation of cyclooxygenase pathway.
【作者单位】： 宁夏医科大学总医院药剂科;宁夏医科大学药学院;宁夏回药现代化工程技术研究中心;宁夏回医药协同创新中心;宁夏医科大学回医药现代化省部共建教育部重点实验室;
【基金】：国家科技支撑计划课题(No.2013BAI11B07) 宁夏回族自治区科技攻关计划项目(No.2012) 大学生创新/创业计划项目(No.201510752008)
【分类号】：R29
，
本文编号：2435966
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