麝香乌龙丸对RA患者外周血miR-146a、miR-130及miR-223表达水平的影响

发布时间：2019-03-10 21:19

【摘要】：目的探讨麝香乌龙丸(人工麝香、制川乌、地龙等)含药血清对类风湿关节炎(RA)患者外周血单个核细胞(PBMCs)中miR-146a、miR-130、miR-223表达水平的影响。方法从30例RA患者血液中提取PBMCs,将细胞分为3组,空白对照组、空白血清组、麝香乌龙丸含药血清组。48 h后提取总RNA,采用实时荧光定量聚合酶链式反应(RT-PCR)法检测3种miR的表达水平。结果与空白血清组相比,麝香乌龙丸含药血清组miR-146a、miR-130、miR-223表达水平显著降低(P0.01)。结论麝香乌龙丸可通过下调PBMCs中miR-223、miR-130、miR-146a表达水平来抑制RA患者炎症。
[Abstract]:Aim to investigate the effect of Shexiang Wulong pills (artificial musk, Radix Polygoni chuanwu, Dilong, etc.) on the expression of miR-146a,miR-130,miR-223 in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA). Methods PBMCs, was extracted from the blood of 30 patients with RA and divided into three groups: blank control group, blank serum group and Shexiang Wulong pill containing serum group. 48 hours later, total RNA, was extracted. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the expression level of three kinds of miR. Results compared with the blank serum group, the expression level of miR-146a,miR-130,miR-223 in the Shexiang Wulong pills group was significantly lower than that in the blank serum group (P0.01). Conclusion Shexiang Wulong Pill can inhibit the inflammation of RA patients by down-regulating the expression of miR-223,miR-130,miR-146a in PBMCs.
【作者单位】：华北理工大学;华北理工大学附属医院;
【基金】：河北省重大医学科研课题资助项目(ZYZ2013017)
【分类号】：R285.6

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