民族药材小一点红中槲皮苷的含量测定

发布时间：2019-03-22 15:21

【摘要】：目的:建立高效液相色谱法测定小一点红中槲皮苷含量的方法。方法:采用单因素试验和正交试验结合数据分析软件优化小一点红药材的提取方法来最大化提取小一点红中的槲皮苷;通过HPLC法测定槲皮苷的含量,采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%乙酸水溶液(20∶80),检测波长351 nm,流速1 m L·min-1,进样量10μL。结果:小一点红的最优提取条件为过40目筛的药材粉末0.5 g,精密加入80%甲醇20 m L,热回流提取30 min,提取1次;槲皮苷在5.541~177.3 mg·L-1(r=0.999 9)线性关系良好;槲皮苷的平均回收率分别为99.87%(RSD 2.0%)。结论:所建立的槲皮苷的含量测定方法简便快捷,专属性强,线性、重复性、精密度和回收率良好,可应用于小一点红的质量控制。
[Abstract]:Objective: To establish a high performance liquid chromatography for the determination of the content of Quercetin in small and small red. Methods: The method of single factor test and orthogonal test combined with data analysis software was used to optimize the extraction method of the small one-to-one red medicinal materials, and the content of the quercetin was determined by HPLC. The content of the mistletoe was determined by HPLC. mobile phase acetonitrile-0.1% aqueous solution of acetic acid (20:80), detection wavelength of 351 nm, flow rate of 1 m L 路 min-1, sample amount of 10. m u.L. Result: The optimum extraction condition of the small one-by-one red is 0.5 g of the medicinal material powder with a 40-mesh sieve,80% of methanol is added in the precision, and the hot reflux is extracted for 30min to extract for 1 time; The linearity of the mistletoe was in the range of 5.541 to 177.3 mg 路 L-1 (r = 0.999 9), and the average recovery of the mistletoe was 99.87% (RSD 2.0%). Conclusion: The method of determination of the content of Quercetin is simple and rapid, the specificity is strong, the linearity, the repeatability, the precision and the recovery rate are good, and the method can be applied to the quality control of the little bit.
【作者单位】：贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室;贵州师范大学贵州省药物质量控制及评价技术工程实验室;贵州师范大学天然药物质量控制研究中心;
【基金】：贵州省高层次创新人才培养项目(黔科合人才(2015)4033号) 贵州省科技厅贵州师范大学联合基金(黔科合LH字[2015]7768号) 贵州省中药材、民族药材质量标准研究项目(DBXZYC52038) 贵州省中药现代化科技产业研究开发专项项目(黔科合中药字[2013]5001号) 贵阳市科技计划项(贵阳市科技重大专项项目)筑科合同[2012401]社-4号
【分类号】：R29;O657.72

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