基于代谢组学慢性心力衰竭肾阳虚证代谢模式的研究

发布时间：2019-03-29 07:04

【摘要】：目的:探讨基于色谱/质谱联用(LC/MS)及模式识别为主的代谢组学技术及其方法,研究慢性心力衰竭肾阳虚证的代谢模式及相应代谢网络的变化。方法:收集正常人以及临床常见慢性心力衰竭肾阳虚证与气阴两虚证病例的尿样,以LC/MS为代谢组学研究方法,检测分析各组间尿样中代谢模式的差异。结果:代谢组学研究表明,与正常组比较慢性心衰组有21种内源性化合物含量与正常组有明显不同;与气阴两虚组比较,慢性心力衰竭肾阳虚组有16种内源性化合物含量与气阴两虚组有明显不同。结论:以代谢模式改变为依据的代谢组学研究方法,通过代谢通路成功地将不同证型加以区分,说明代谢组学技术及其方法研究中医学证本质的思路及方法可行,从代谢模式的角度阐述中医证本质是中医现代化可实践和深化的技术和方法学体系。
[Abstract]:Aim: to study the metabolic pattern and metabolic network of kidney yang deficiency syndrome in chronic heart failure (CHF) based on chromatography / mass spectrometry (LC/MS) and pattern recognition. Methods: urine samples were collected from normal subjects and patients with chronic heart failure such as kidney-yang deficiency syndrome and qi-yin deficiency syndrome. LC/MS was used as a metabonomics method to detect and analyze the difference of metabolic patterns in urine samples among the three groups. Results: the contents of 21 endogenous compounds in chronic heart failure group were significantly different from those in normal group. Compared with the Qi-yin deficiency group, the contents of 16 endogenous compounds in the chronic heart failure group were significantly different from those in the Qi-yin deficiency group. Conclusion: the metabonomics research method based on the change of metabolic pattern successfully distinguishes different syndrome types through metabolic pathway, which shows that the idea and method of studying the essence of TCM syndrome by metabonomics and its methods are feasible. From the point of view of metabolic mode, the essence of TCM syndrome is a technical and methodological system which can be practiced and deepened in the modernization of traditional Chinese medicine.
【作者单位】：宁夏医科大学;上海中医药大学附属曙光医院;
【基金】：国家自然科学基金资助项目(30572286)-基于代谢组学的肾阳虚证本质的研究
【分类号】：R259

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