揉髌手法对兔膝骨关节炎模型血清IL-1β、IL-6、IL-13、IL-26、TNF-α含量的影响
发布时间：2019-04-04 17:17
【摘要】：目的:观察揉髌手法对兔膝骨关节炎模型血清IL-1β、IL-6、IL-13、IL-26、TNF-α等细胞因子含量的影响,探寻该手法在膝骨关节炎治疗中的获效机制,为膝骨关节炎的临床诊治提供理论支持。方法:运用随机数字表法将52只新西兰大白兔分组:手法组、对照组、假模组和正常组,每组均为13只。手法组、对照组共26只新西兰大白兔施行右臀下肢静脉结扎手术。假模组13只新西兰大白兔则手术显露出上述静脉,但不予结扎。剩余13只兔子是正常组,不做上述相关处理。造模1周后,研究者开始针对手法组进行揉髌手法治疗。为减小实验误差,本次研究揉髌手法采用专人施术。手法治疗方案:隔日1次,5周共17次,10min/次,另外三组不做任何治疗。首次静脉采血时间为造模第1周末,第2次静脉采血时间是第12周末。沿耳缘静脉缓慢抽取2ml血液,然后出针。采血操作严格遵守无菌原则,减少采血后实验动物的感染率,降低微生物代谢对实验造成的影响。将采出的血液样本注入事先准备好的无菌EP管中,处理完的EP管放置于保温箱中。用ELISA法检测IL-1β、IL-6、IL-13、IL-26、TNF-α的含量。在完成第2次静脉采血后(第12周末),使用注射器从耳缘静脉注入空气,空气栓塞处死所有兔子,取右胫骨平台内侧软骨送病理检测以评估软骨退变情况。结果:1、手法组兔膝关节软骨Mankin,s评分为轻度损伤,明显优于对照组的中重度损伤;2、12周末,手法组血清中IL-1β、IL-6、IL-13、IL-26、TNF-α含量均低于对照组,组间比较有统计学差异。结论:1、膝骨关节炎的骨内高压和骨静脉瘀血,导致炎症因子积累,致关节软骨细胞和软骨基质的丢失,加速软骨退变。此外,膝关节周围局部创伤,在一定程度上影响关节软骨的退变进程。2、揉髌手法可明显降低动物模型血清IL-1β、IL-6、IL-13、IL-26、TNF-α的含量,缓解了关节软骨退变,这可能是该手法在临床上治疗膝OA获效机制之一。
[Abstract]:Objective: to observe the effect of patellar kneading manipulation on the content of serum IL-1 尾, IL-6,IL-13,IL-26,TNF- 伪 and other cytokines in rabbit knee osteoarthritis model, and to explore the effective mechanism of patellar kneading manipulation in the treatment of knee osteoarthritis. To provide theoretical support for the clinical diagnosis and treatment of knee osteoarthritis. Methods: fifty-two New Zealand white rabbits were divided into three groups: manipulation group, control group, sham model group and normal group, 13 rabbits in each group were randomly divided into three groups: manipulation group, control group, sham model group and normal group. Manipulation group, control group 26 New Zealand rabbits underwent right gluteal and lower limb vein ligation. In the sham model group, 13 New Zealand white rabbits showed the above vein, but did not ligate it. The remaining 13 rabbits were normal and were not treated above. A week later, the researchers began kneading patellar manipulations for the manipulative group. In order to reduce the experimental error, the patellar kneading technique was used in this study. Manual therapy regimen: every other day, 5 weeks, 17 times, 10min/, the other three groups without any treatment. The first venous sampling time was at the end of the model, and the second vein sampling time was at the end of the 12th week. The 2ml blood was drawn slowly along the auricular vein and then the needle was given. The operation of blood collection strictly obeys the principle of asepsis, reduces the infection rate of experimental animals after blood sampling, and reduces the influence of microbial metabolism on the experiment. The collected blood samples were injected into the pre-prepared sterile EP tube, and the treated EP tube was placed in the insulation box. The contents of IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 were determined by ELISA. At the end of the 12th week, air was injected into the auricular vein with a syringe, and all rabbits were killed by air embolization. The medial cartilage of the right tibial plateau was taken for pathological examination to evaluate the degeneration of cartilage. Results: 1. The Mankin, s of knee joint cartilage in the manipulation group was classified as mild injury, which was obviously superior to the moderate and severe injury in the control group. At the end of 2 and 12 weeks, the serum levels of IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 in the manipulation group were lower than those in the control group, and there was a statistical difference between the two groups. Conclusions: 1. Intraosseous hypertension and blood stasis of bone vein in knee osteoarthritis lead to accumulation of inflammatory factors, loss of articular chondrocytes and cartilage matrix, and acceleration of cartilage degeneration. 2. In addition, the local trauma around the knee joint affects the degeneration of articular cartilage to a certain extent. 2. Patellar manipulation can significantly reduce the content of serum IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 in the animal model. Remission of articular cartilage degeneration may be one of the effective mechanisms of this technique in the clinical treatment of knee OA.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R244.1;R-332
本文编号：2454009
[Abstract]:Objective: to observe the effect of patellar kneading manipulation on the content of serum IL-1 尾, IL-6,IL-13,IL-26,TNF- 伪 and other cytokines in rabbit knee osteoarthritis model, and to explore the effective mechanism of patellar kneading manipulation in the treatment of knee osteoarthritis. To provide theoretical support for the clinical diagnosis and treatment of knee osteoarthritis. Methods: fifty-two New Zealand white rabbits were divided into three groups: manipulation group, control group, sham model group and normal group, 13 rabbits in each group were randomly divided into three groups: manipulation group, control group, sham model group and normal group. Manipulation group, control group 26 New Zealand rabbits underwent right gluteal and lower limb vein ligation. In the sham model group, 13 New Zealand white rabbits showed the above vein, but did not ligate it. The remaining 13 rabbits were normal and were not treated above. A week later, the researchers began kneading patellar manipulations for the manipulative group. In order to reduce the experimental error, the patellar kneading technique was used in this study. Manual therapy regimen: every other day, 5 weeks, 17 times, 10min/, the other three groups without any treatment. The first venous sampling time was at the end of the model, and the second vein sampling time was at the end of the 12th week. The 2ml blood was drawn slowly along the auricular vein and then the needle was given. The operation of blood collection strictly obeys the principle of asepsis, reduces the infection rate of experimental animals after blood sampling, and reduces the influence of microbial metabolism on the experiment. The collected blood samples were injected into the pre-prepared sterile EP tube, and the treated EP tube was placed in the insulation box. The contents of IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 were determined by ELISA. At the end of the 12th week, air was injected into the auricular vein with a syringe, and all rabbits were killed by air embolization. The medial cartilage of the right tibial plateau was taken for pathological examination to evaluate the degeneration of cartilage. Results: 1. The Mankin, s of knee joint cartilage in the manipulation group was classified as mild injury, which was obviously superior to the moderate and severe injury in the control group. At the end of 2 and 12 weeks, the serum levels of IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 in the manipulation group were lower than those in the control group, and there was a statistical difference between the two groups. Conclusions: 1. Intraosseous hypertension and blood stasis of bone vein in knee osteoarthritis lead to accumulation of inflammatory factors, loss of articular chondrocytes and cartilage matrix, and acceleration of cartilage degeneration. 2. In addition, the local trauma around the knee joint affects the degeneration of articular cartilage to a certain extent. 2. Patellar manipulation can significantly reduce the content of serum IL-1 尾 and IL-6,IL-13,IL-26,TNF- 伪 in the animal model. Remission of articular cartilage degeneration may be one of the effective mechanisms of this technique in the clinical treatment of knee OA.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R244.1;R-332
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