藏药镰形棘豆醇提物酸碱分离及抗小鼠Lewis肺癌活性初步筛选

发布时间：2019-04-11 07:51

【摘要】：目的筛选藏药镰形棘豆醇提物中对Lewis肺癌荷瘤小鼠具有抑瘤作用的活性部位。方法利用酸碱处理对镰形棘豆醇提物进行分离,并对所得碱性部位(AlF)和酸性部位(AcF)进行定性、定量分析。建立Lewis肺癌C57BL/6小鼠模型,随机分为模型组,环磷酰胺(CTX)组(20 mg·kg~(-1)),AlF高、低剂量组(180,90 mg·kg~(-1)),AcF高、低剂量组(180,90 mg·kg~(-1)),连续给药10 d;通过测定小鼠瘤体质量、抑瘤率、胸腺指数、脾指数来比较AlF、AcF的抑瘤作用;HE染色,显微镜下观察各组肿瘤细胞的形态学变化。结果 AcF主要含黄酮类成分,含量为18.9%;AlF主要为生物碱成分,含量为64.1%。AcF高剂量组降低雌性、雄性小鼠瘤体质量的作用明显,与模型组比较,差异有统计学意义(P0.01,P0.05),抑瘤率分别为36.3%、32.8%;AlF高剂量组降低雌性小鼠瘤体质量的作用明显,与模型组比较,差异有统计学意义(P0.05),抑瘤率为29.7%。与模型组比较,CTX组小鼠的脾脏、胸腺指数均明显降低,差异有统计学意义(P0.05,P0.01);而AcF、AlF各剂量组与模型组比较,脾脏、胸腺指数差异均无统计学意义(P0.05)。肿瘤组织病理形态学观察显示AcF、AlF各给药组肿瘤细胞出现不同程度的坏死。结论镰形棘豆醇提物AlF、AcF均对Lewis肺癌荷瘤小鼠具有明显的抗肿瘤作用,黄酮类成分的作用比生物碱略强,其抗肿瘤机制还有待进一步研究。
[Abstract]:Objective to screen the active parts of alcohol extract from Tibetan medicine against Lewis lung cancer-bearing mice. Methods the alcohol extract of sickle bean was separated by acid-base treatment, and the (AlF) and (AcF) were analyzed qualitatively and quantitatively. C57BL/6 mice model of Lewis lung cancer was established and randomly divided into model group, cyclophosphamide (CTX) group (20 mg kg~ (- 1), AlF high), low dose group (180, 90 mg kg~ (- 1), AcF), low dose group) (180, 90 mg kg~ (- 1),). Continuous administration for 10 days; The tumor weight, tumor inhibition rate, thymus index and spleen index of mice were measured to compare the antitumor effect of AlF,AcF, and the morphological changes of tumor cells in each group were observed under microscope by HE staining. Results AcF was mainly composed of flavonoids, the content of which was 18.9%. The content of AlF was mainly alkaloid in the high-dose 64.1%.AcF group. The tumor weight of male mice was significantly lower than that of the model group (P0.01, P0.05), and the tumor weight of male mice was significantly lower than that of the model group (P0.01, P0.05). The tumor inhibition rate was 36.3% and 32.8% respectively. Compared with the model group, the high dose of AlF could decrease the tumor weight of female mice significantly (P0.05), and the tumor inhibition rate was 29.7%. Compared with the model group, the index of spleen and thymus in CTX group decreased significantly (P 0.05, P0.01). Compared with the model group, there was no significant difference in spleen and thymus index between each dose group of AcF,AlF and the model group (P0.05). Pathological observation of tumor tissue showed different degree of necrosis of tumor cells in each group of AcF,AlF administration. Conclusion the alcohol extract AlF,AcF from sickle bean has obvious antitumor effect on Lewis lung cancer-bearing mice. The effect of flavonoids is slightly stronger than that of alkaloids, and its anti-tumor mechanism needs to be further studied.
【作者单位】：南京中医药大学药学院;南京中医药大学药用菌与中药生物技术研究所;江苏省中药炮制重点实验室国家中医药管理局中药炮制标准重点研究室;
【基金】：国家自然科学基金项目(81473420,30902012) 江苏高校优势学科建设工程资助项目(PAPD) 江苏高校品牌专业建设工程资助项目(PPZY2015A070)
【分类号】：R29

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