新加阳和汤对强直性脊柱炎细胞因子及磷酸化STAT3表达的影响

发布时间：2019-05-18 10:35

【摘要】：目的：通过检测白介素6(IL-6)、白介素17(IL-17)、白介素21(IL-21)在血清中含量及磷酸化信号转导及转录激活因子3 (pSTAT3)在关节组织的表达情况,探讨新加阳和汤调节免疫、抑制炎症作用,初步从细胞、分子水平分析其作用机制。方法：取48只Balb/c小鼠,随机分为6组,分别是正常组、模型组、阳性药组、中药低、中、高剂量组。采用蛋白聚糖±弗氏佐剂腹腔注射的方法造模,一周后强化造模。各给药组均于造模结束后一周开始给药,每日1次,直到灌胃第91天后球后静脉放血处死小鼠,解剖分离取一侧膝关节及脊柱。测血清IL-6、IL-17、IL-21的浓度,免疫组化测定膝关节及脊柱的磷酸化STAT3的含量,并观察脊柱及膝关节的病理变化。结果：与正常组相比,模型组IL-6、IL-17、IL-21、pSTAT3均显著增加(P0.05),而给予药物后,中药各剂量组的IL-6、17、21及pSTAT3含量下降,与模型组相比,差异有显著或非常显著的意义(P0.05,P0.01)。结论：1.模型组IL-6、IL-17、IL-21及pSTAT3的表达高于正常组,关节病理有炎性浸润,说明细胞因子IL-6、17、21及pSTAT3参与了AS的发生。2.新加阳和汤能够降低血清IL-6、17、21的含量,抑制pSTAT3的表达,表明新加阳和汤治疗强直性脊柱炎可以通过抑制IL-6、17、21及pSTAT3的表达。
[Abstract]:Objective: to detect the contents of IL-6 (IL-6), IL-17 (IL-17) and IL-21 (IL-21) in serum and the expression of phosphorylation signal transduction and activating factor 3 (pSTAT3) in joint tissue. To explore the effect of Xinjiahe decoction on regulating immunity and inhibiting inflammation, and to analyze its mechanism at cell and molecular level. Methods: 48 Balb/c mice were randomly divided into 6 groups: normal group, model group, positive drug group, low, middle and high dose groups. The model was established by intraabdominal injection of proteoglycan 卤Freund adjuvant, and the model was strengthened one week later. The mice in each administration group were given once a day one week after the end of the model, until the 91st day after intragastric administration, the mice were killed by bloodletting behind the bulbar vein, and a knee joint and spine were dissected and separated. The concentration of serum IL-6,IL-17,IL-21 was measured, the content of phosphorylated STAT3 in knee joint and spine was measured by immunohistochemistry, and the pathological changes of spine and knee joint were observed. Results: compared with the normal group, the IL-6,IL-17,IL-21,pSTAT3 of the model group was significantly increased (P 0.05). After administration of the drug, the contents of IL-6,17,21 and pSTAT3 in each dose group of traditional Chinese medicine decreased, compared with the model group. The difference was significant or very significant (P 0.05, P 0.01). Conclusion: 1. The expression of IL-6,IL-17,IL-21 and pSTAT3 in the model group was higher than that in the normal group, and inflammatory infiltration was found in the joint pathology, indicating that cytokines IL-6,17,21 and pSTAT3 were involved in the occurrence of AS. 2. Singapore Yanghe decoction can reduce the content of serum IL-6,17,21 and inhibit the expression of pSTAT3, which indicates that Xinjiayang he decoction can inhibit the expression of IL-6,17,21 and pSTAT3 in the treatment of ankylosing spondylitis.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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