穴位埋线对血管性痴呆大鼠海马CA1区p53蛋白表达的影响

发布时间：2019-05-22 23:03

【摘要】：目的观察穴位埋线对血管性痴呆(VD)大鼠海马CA1区p53蛋白表达的影响。方法取SD大鼠随机分成假手术组、VD模型组、穴位埋线组、尼莫地平组,采用改良的Pulsinelli四血管闭塞法(4-VO)建立VD大鼠模型,两个治疗组分别施行穴位埋线和尼莫地平治疗,连续15 d。水迷宫行为学测试后,断头处死大鼠,采集海马CA1区,用免疫组织化学法检测其p53表达,并取大脑皮质做冠状位病理切片进行HE染色,观察并比较各组大鼠海马CA1区p53和大脑皮质神经元损伤程度。结果假手术组海马CA1区未见p53阳性细胞,穴位埋线组及尼莫地平组海马CA1区p53阳性细胞平均灰度值均明显高于VD模型组(P0.05),大脑皮质神经元的损伤和变性减轻且数量增加。结论穴位埋线可明显降低海马CA1区p53蛋白的表达,抑制VD大鼠脑损伤引起的细胞凋亡,对脑组织起保护作用,这可能是其治疗VD的作用机制之一。
[Abstract]:Objective to observe the effect of acupoint catgut embedding on the expression of p53 protein in hippocampal CA1 region of vascular dementia (VD) rats. Methods SD rats were randomly divided into three groups: sham operation group, VD model group, acupoint embedding group and nimodipine group. The VD rat model was established by modified Pulsinelli four-vessel occlusive method (4-VO). The two treatment groups were treated with catgut embedding at acupoints and nimodipine for 15 days. After the water maze behavior test, the rats were decapitated and the hippocampal CA1 area was collected. The expression of p53 was detected by immunohistochemistry, and the coronal pathological sections of the cerebral cortex were taken for HE staining. The injury degree of p53 and cerebral cortical neurons in hippocampal CA1 region of rats in each group was observed and compared. Results there were no p53 positive cells in hippocampal CA1 area of sham operation group. The average gray value of p53 positive cells in hippocampal CA1 area of acupoint embedding group and nimodipine group was significantly higher than that of VD model group (P 0.05). The damage and degeneration of cortical neurons were alleviated and the number of neurons increased. Conclusion Acupoint catgut embedding can significantly decrease the expression of p53 protein in hippocampal CA1 region, inhibit the apoptosis induced by brain injury in VD rats, and play a protective role in brain tissue, which may be one of the mechanisms of its treatment of VD.
【作者单位】：贵阳护理职业学院基础护理教研室;贵阳中医学院解剖学教研室;
【基金】：贵州省教育厅自然科学研究项目(No.黔教科2010050)
【分类号】：R245

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