高脂诱导自发性高血压大鼠脂肪组织SIRT1的表达下调及清肝滋肾法的调控作用
发布时间：2019-05-29 13:33
【摘要】：目的：探讨清肝滋肾中药(针箭颗粒Ⅱ号)通过上调内脏脂肪组织中SIRT1的表达改善高血压代谢紊乱的作用及机制。方法：通过高脂饮食诱导获得自发性高血压大鼠代谢紊乱模型。选取17只4周龄的雄性自发性高血压大鼠(SHR),分为高脂饮食组12只和正常饮食组5只,正常饮食组给予基础饲料,高脂饮食组给予高脂饲料。高脂饮食组则随机分为针箭颗粒组6只(22.5g/kg/d,灌胃)和模型对照组6只(同体积蒸馏水,灌胃)。每周测量体重、血压2次,根据体重改变药物给药量。给药9周后处死,获取大鼠内脏脂肪(附睾周围)并称重,部分内脏脂肪分离脂肪细胞,部分提取mRNA和蛋白,部分给予4%多聚甲醛固定HE染色。1)通过定期监测SHR鼠血压、体重,评估SHR大鼠代谢情况。2)采用RT-PCR和Western Blot法测定内脏脂肪组织SIRT1 mRNA-及蛋白、裂解酶Caspase-1蛋白的表达水平,评估SIRT1表达水平和活性的变化；结果：1.清肝滋肾中药(针箭颗粒Ⅱ号)对SHR大鼠体重的影响：基线时SHR大鼠三组之间体重无明显差异(P0.05)；干预9周后模型组体重显著高于对照组(P0.01),针箭颗粒组体重显著低于模型组(P0.01),对照组与针箭颗粒组之间无显著差异(P0.05)。2.清肝滋肾中药(针箭颗粒Ⅱ号)对SHR大鼠血压的影响：治疗前模型组SBP高于对照组(P0.05),其余组间无显著差异(P0.05)；三组间DBP无明显差异(P0.05)。干预9周后,模型组与对照组之间血压(SBP、DBP)无明显差异,针箭颗粒组虽有下降趋势但无统计学差异(P0.05)。3.清肝滋肾中药(针箭颗粒Ⅱ号)对SHR大鼠内脏脂肪(附睾周围)的影响3.1各组附睾周围脂肪重量及其体脂比的比较：1)干预9周后模型组附睾周围脂肪含量高于对照组(P0.05),但针箭颗粒组与模型组间无明显差异(P0.05)；2)干预9周后模型组体脂比显著高于对照组(P0.01),但针箭颗粒组与模型组间无明显差异(P0.05)。3.2镜下评估附睾周围脂肪体积及脂滴含量：模型组与对照组相比,脂肪细胞体积增大,脂滴含量增加；针箭颗粒组与模型组相比,脂肪细胞体积减小,脂滴含量减少。4.清肝滋肾中药(针箭颗粒Ⅱ号)对SHR大鼠内脏脂肪(附睾周围)组织SIRT1表达及活性变化的影响4.1清肝滋肾中药(针箭颗粒Ⅱ号)对附睾周围脂肪组织中SIRT1 mRNA表达的影响：模型组SIRT1 mRNA的表达显著低于对照组(P0.01),而针箭颗粒组SIRT1 mRNA的表达高于模型组(P0.05)。4.2清肝滋肾中药(针箭颗粒Ⅱ号)对附睾周围脂肪组织中SIRT1蛋白表达的影响：干预9周后,模型组SIRT1蛋白表达显著低于对照组(P0.01),针箭颗粒组SIRT1蛋白表达则高于模型组(P0.05)。4.3清肝滋肾中药(针箭颗粒Ⅱ号)对附睾周围脂肪组织中裂解酶Caspase1蛋白表达的影响：干预9周后三组之间Caspase1蛋白表达水平无显著差异(P0.05)。结论：1.清肝滋肾中药(针箭颗粒Ⅱ号)可降低SHR合并代谢紊乱大鼠体重,减少内脏脂肪的堆积。2.清肝滋肾中药(针箭颗粒Ⅱ号)可能是通过上调内脏脂肪组织中SIRT1的表达来改善高血压代谢紊乱。
[Abstract]:Objective: To study the effect and mechanism of the expression of SIRT1 in visceral fat tissue by regulating the expression of SIRT1 in visceral fat tissue. Methods: The metabolic disorder model of spontaneously hypertensive rats was induced by high-fat diet. 17-week-old male spontaneously hypertensive rats (SHR) were selected and divided into two groups: high-fat diet group (n = 12) and normal diet group (n = 5). The normal diet group was given the basic feed and the high-fat diet group was given a high-fat diet. The high-fat diet group was randomly divided into 6 groups (22.5 g/ kg/ day, gavage) and 6 model control groups (with the same volume of distilled water and intragastric administration). Body weight was measured weekly, blood pressure was 2 times, and the amount of drug was changed according to body weight. After 9 weeks of administration, the visceral fat (around the epididymis) of the rat was obtained and weighed, the fat cells were isolated from the part of the visceral fat, the mRNA and the protein were partially extracted, and the part was given 4% paraformaldehyde fixed HE staining.1) The blood pressure and body weight of the SHR rats were monitored regularly by periodically monitoring the blood pressure and body weight of the SHR rats, (2) The expression level of SIRT1 mRNA and protein and caspase-1 protein in visceral fat tissue were determined by RT-PCR and Western Blot method, and the expression level and activity of SIRT1 were assessed. The effect of Qinggan Zishen traditional Chinese medicine (No. 鈪，
本文编号：2487970
[Abstract]:Objective: To study the effect and mechanism of the expression of SIRT1 in visceral fat tissue by regulating the expression of SIRT1 in visceral fat tissue. Methods: The metabolic disorder model of spontaneously hypertensive rats was induced by high-fat diet. 17-week-old male spontaneously hypertensive rats (SHR) were selected and divided into two groups: high-fat diet group (n = 12) and normal diet group (n = 5). The normal diet group was given the basic feed and the high-fat diet group was given a high-fat diet. The high-fat diet group was randomly divided into 6 groups (22.5 g/ kg/ day, gavage) and 6 model control groups (with the same volume of distilled water and intragastric administration). Body weight was measured weekly, blood pressure was 2 times, and the amount of drug was changed according to body weight. After 9 weeks of administration, the visceral fat (around the epididymis) of the rat was obtained and weighed, the fat cells were isolated from the part of the visceral fat, the mRNA and the protein were partially extracted, and the part was given 4% paraformaldehyde fixed HE staining.1) The blood pressure and body weight of the SHR rats were monitored regularly by periodically monitoring the blood pressure and body weight of the SHR rats, (2) The expression level of SIRT1 mRNA and protein and caspase-1 protein in visceral fat tissue were determined by RT-PCR and Western Blot method, and the expression level and activity of SIRT1 were assessed. The effect of Qinggan Zishen traditional Chinese medicine (No. 鈪，
本文编号：2487970
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