复方芪鹰颗粒对糖尿病周围神经病变大鼠Hsp70表达的影响

发布时间：2019-06-07 17:16

【摘要】：目的观察复方芪鹰颗粒对糖尿病周围神经病变(DPN)大鼠坐骨神经Hsp70表达及坐骨神经损伤纤维的影响,探讨其机制。方法从60只Wistar大鼠中随机取出10只为正常组,50只大鼠造DPN模型,将成模43只大鼠随机分为模型组(11只)、复方芪鹰颗粒低剂量组(11只)、复方芪鹰颗粒中剂量组(11只)、复方芪鹰颗粒高剂量组(10只),实验过程中,模型组死亡3只,复方芪鹰颗粒低剂量组死亡1只。复方芪鹰颗粒低、中、高剂量组分别为1.17、2.34、4.68 g/kg,模型组、正常组均灌以相应容积的蒸馏水,1次/d。治疗4周后,观察各组Hsp70表达、坐骨神经损伤纤维变化。结果复方芪鹰颗粒各剂量组及模型组坐骨神经中Hsp70表达与正常组比较,差异有高度统计学意义(P0.01);复方芪鹰颗粒中、高剂量组坐骨神经中Hsp70表达与模型组比较,差异有高度统计学意义(P0.01),而中剂量及高剂量组间比较差异无统计学意义(P0.05);复方芪鹰颗粒各剂量组及模型组与正常组在在坐骨神经损伤纤维百分比方面比较,差异有高度统计学意义(P0.01);复方芪鹰颗粒低、中、高剂量组与模型组在坐骨神经损伤纤维百分比方面比较,差异有高度统计学意义(P0.01)。结论复方芪鹰颗粒可能通过调节DPN大鼠坐骨神经Hsp70水平,从而改善受损坐骨神经。
[Abstract]:Objective to observe the effect of compound Qiying granule on the expression of Hsp70 and injured fibers in sciatica of diabetic peripheral neuropathy (DPN) rats and to explore its mechanism. Methods 10 Wistar rats were randomly selected from 60 DPN rats as normal group and 50 rats as DPN model. 43 rats were randomly divided into model group (n = 11) and compound Qiying granule low dose group (n = 11), and the model group (n = 11) was randomly divided into model group (n = 11) and compound Qiying granule low dose group (n = 11). In the middle dose group (n = 11) and the high dose group (n = 10), 3 rats died in the model group and 1 in the low dose group. The low, middle and high dose groups of compound Qiying granule were 1.17, 2.34 and 4.68 g 路kg ~ (- 1), respectively. the model group and the normal group were treated with distilled water of the corresponding volume once a day. After 4 weeks of treatment, the expression of Hsp70 and the changes of nerve injured fibers in each group were observed. Results the expression of Hsp70 in the sciatica of each dose group and model group was significantly different from that of the normal group (P 0.01). Compared with the model group, the expression of Hsp70 in the sciatica of the high dose group was significantly different from that of the model group (P 0.01), but there was no significant difference between the middle dose group and the high dose group (P 0.05). Compared with the normal group, the percentage of fibers in each dose group and model group of compound Qiying granule was significantly different from that in the normal group (P 0.01). Compared with the model group, the percentage of fibers in the high dose group and the model group was significantly different from that in the model group (P 0.01), and the percentage of fibers in the high dose group was significantly higher than that in the model group (P 0.01). Conclusion compound Qiying granule may improve the injured sciatica by regulating the level of Hsp70 in the sciatica of DPN rats.
【作者单位】：新疆维吾尔自治区中医医院老年病科;新疆维吾尔自治区中医医院药剂科;新疆医科大学研究生院;
【基金】：新疆医科大学附属中医医院院级课题(ZYY201428)
【分类号】：R259;R277.7

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