基于病证相关的“心与小肠相表里”实验研究
发布时间：2019-06-10 19:25
【摘要】：目的:探讨不同中医证候状态下,心与小肠功能变化和相互关联。方法:将40只家兔随机分正常组、心火亢盛证组、肾实热证组、小肠实热证组,每组10只。家兔耳缘静脉注射强毒力的大肠杆菌建立心火亢盛证模型;家兔耳缘静脉注射小牛血清建立肾实热证模型;经口腔灌饲辣椒汁、白酒混合液建立小肠实热证模型。观察各组家兔心脏超声实时影像、血清心肌酶谱、血清尿素氮与肌酐、酚红排泄率、小肠吸收能力、空肠平滑肌张力与收缩频率。结果:各组家兔心功能比较:除舒张末期左室后壁厚度各组比较,无显著性差异外,心火亢盛证组与小肠实热证组其余各指标如心率、左室舒张末期内径、左室收缩末期内径、左室射血分数、左室短轴缩短率,血清磷酸肌酸激酶(creatine phosphokinase,CK)、肌酸激酶同工酶、乳酸脱氢酶比较,差异均有统计学意义(P0.05);而与肾实热证组无显著差异(P0.05);与小肠实热证组相比,肾实热证组各指标存在显著差异性(P0.05)。各组家兔肾功能比较:心火亢盛证组血清尿素氮、肌酐与小肠实热证组比较,差异均有统计学意义(P0.05),而与肾实热证组无显著差异(P0.05);与小肠实热证组相比,肾实热证组各指标差异显著(P0.05);酚红排泄实验显示,与正常组比较,3个时间段各模型组家兔酚红排泄率均下降;心火亢盛证组与小肠实热证组比较,差异有统计学意义(P0.05),而与肾实热证组无显著差异(P0.05);与小肠实热证组比较,肾实热证组各指标差异显著(P0.05)。小肠功能观察,灌胃后血中葡萄糖浓度心火亢盛组、肾实热组之间差异亦不显著(P0.05),二者与小肠实热证组比较,差异有统计学意义(P0.05);3个模型组空肠离体平滑肌张力和肌收缩频率也均较正常组增高,心火亢盛证组与小肠实热证组比较,差异有统计学意义(P0.05),而与肾实热证组无显著差异(P0.05),与小肠实热证组相比,肾实热证组各指标存在显著差异性(P0.05)。结论:在中医相关证候状态下,心与肾功能变化及其联系,较之小肠似乎更为紧密,"心与小肠相表里"之实质大致应该更多的从现代解剖学心与肾之间的关联去探讨。
[Abstract]:Objective: to investigate the changes and correlation between heart and small intestine function under different TCM syndromes. Methods: forty rabbits were randomly divided into normal group, heart fire hyperactivity syndrome group, kidney excess heat syndrome group and small intestine heat syndrome group with 10 rabbits in each group. The model of hyperactivity of heart fire was established by intravenous injection of Escherichia coli at the ear edge of rabbits, the model of kidney excess heat syndrome was established by intravenous injection of calf serum into the ear edge of rabbits, and the model of small intestine heat syndrome was established by oral administration of chili juice and liquor mixture. The real-time ultrasound images, serum myocardial zymogram, serum urea nitrogen and creatine excretion rate, phenol red excretion rate, intestinal absorption capacity, tension and contraction frequency of Jejunum smooth muscle were observed. Results: comparison of cardiac function in each group: except for the thickness of left ventricular posterior wall at the end of diastolic stage, there was no significant difference between the hyperactivity syndrome group and the small intestine heat syndrome group, such as heart rate, left ventricular end-diastolic diameter and left ventricular end-systolic diameter. Left ventricular ejection fraction, left ventricular short axis shortening rate, serum creatine kinase (creatine phosphokinase,CK), creatine kinase isoenzyme and lactic dehydrogenase were significantly different (P 0.05). There was no significant difference between the kidney excess heat syndrome group and the kidney excess heat syndrome group (P 0.05), and there was significant difference in each index between the kidney excess heat syndrome group and the small intestine excess heat syndrome group (P 0.05). Comparison of renal function in each group: there was significant difference in serum urea nitrogen and creatinine between Xinhuo Sheng syndrome group and small intestine heat syndrome group (P 0.05), but there was no significant difference between Xinhuo Sheng syndrome group and kidney excess heat syndrome group (P 0.05), but there was no significant difference between Xinhuo Sheng syndrome group and small intestine heat syndrome group (P 0.05). Compared with the small intestine heat syndrome group, the indexes of the kidney excess heat syndrome group were significantly different (P 0.05), and the phenol red excretion test showed that compared with the normal group, the phenol red excretion rate of rabbits in each model group decreased in three time periods. There was significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference with the kidney excess heat syndrome group (P 0.05). The observation of small intestinal function, the concentration of glucose in blood after gastric perfusion was not significantly different between Xinhuo hyperactivity group and renal hyperpyretic group (P 0.05), and there was significant difference between the two groups (P 0.05). The tension and muscle contraction frequency of isolated Jejunum smooth muscle in the three model groups were also higher than those in the normal group. There was significant difference between the heart fire hyperactivity syndrome group and the small intestine heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the kidney excess heat syndrome group (P 0.05). Compared with the small intestine heat syndrome group, there was significant difference in each index in the kidney excess heat syndrome group (P 0.05). Conclusion: under the condition of TCM related syndromes, the changes of heart and kidney function and their relationship seem to be closer than those of small intestine. The essence of "heart and small intestine phase table" should be discussed more from the relationship between heart and kidney in modern anatomy.
【作者单位】： 首都医科大学附属北京中医医院;
【基金】：国家自然科学基金项目(81273614) 北京市自然科学基金项目(7152070)
【分类号】：R228
本文编号：2496676
[Abstract]:Objective: to investigate the changes and correlation between heart and small intestine function under different TCM syndromes. Methods: forty rabbits were randomly divided into normal group, heart fire hyperactivity syndrome group, kidney excess heat syndrome group and small intestine heat syndrome group with 10 rabbits in each group. The model of hyperactivity of heart fire was established by intravenous injection of Escherichia coli at the ear edge of rabbits, the model of kidney excess heat syndrome was established by intravenous injection of calf serum into the ear edge of rabbits, and the model of small intestine heat syndrome was established by oral administration of chili juice and liquor mixture. The real-time ultrasound images, serum myocardial zymogram, serum urea nitrogen and creatine excretion rate, phenol red excretion rate, intestinal absorption capacity, tension and contraction frequency of Jejunum smooth muscle were observed. Results: comparison of cardiac function in each group: except for the thickness of left ventricular posterior wall at the end of diastolic stage, there was no significant difference between the hyperactivity syndrome group and the small intestine heat syndrome group, such as heart rate, left ventricular end-diastolic diameter and left ventricular end-systolic diameter. Left ventricular ejection fraction, left ventricular short axis shortening rate, serum creatine kinase (creatine phosphokinase,CK), creatine kinase isoenzyme and lactic dehydrogenase were significantly different (P 0.05). There was no significant difference between the kidney excess heat syndrome group and the kidney excess heat syndrome group (P 0.05), and there was significant difference in each index between the kidney excess heat syndrome group and the small intestine excess heat syndrome group (P 0.05). Comparison of renal function in each group: there was significant difference in serum urea nitrogen and creatinine between Xinhuo Sheng syndrome group and small intestine heat syndrome group (P 0.05), but there was no significant difference between Xinhuo Sheng syndrome group and kidney excess heat syndrome group (P 0.05), but there was no significant difference between Xinhuo Sheng syndrome group and small intestine heat syndrome group (P 0.05). Compared with the small intestine heat syndrome group, the indexes of the kidney excess heat syndrome group were significantly different (P 0.05), and the phenol red excretion test showed that compared with the normal group, the phenol red excretion rate of rabbits in each model group decreased in three time periods. There was significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the small intestine excess heat syndrome group (P 0.05), but there was no significant difference with the kidney excess heat syndrome group (P 0.05). The observation of small intestinal function, the concentration of glucose in blood after gastric perfusion was not significantly different between Xinhuo hyperactivity group and renal hyperpyretic group (P 0.05), and there was significant difference between the two groups (P 0.05). The tension and muscle contraction frequency of isolated Jejunum smooth muscle in the three model groups were also higher than those in the normal group. There was significant difference between the heart fire hyperactivity syndrome group and the small intestine heat syndrome group (P 0.05), but there was no significant difference between the heart fire hyperactivity syndrome group and the kidney excess heat syndrome group (P 0.05). Compared with the small intestine heat syndrome group, there was significant difference in each index in the kidney excess heat syndrome group (P 0.05). Conclusion: under the condition of TCM related syndromes, the changes of heart and kidney function and their relationship seem to be closer than those of small intestine. The essence of "heart and small intestine phase table" should be discussed more from the relationship between heart and kidney in modern anatomy.
【作者单位】： 首都医科大学附属北京中医医院;
【基金】：国家自然科学基金项目(81273614) 北京市自然科学基金项目(7152070)
【分类号】：R228
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