脾虚痰浊证巴马小型猪心肌糖代谢相关分子的变化特点
发布时间：2019-06-27 17:51
【摘要】：目的:探讨脾虚痰浊证巴马小型猪心肌组织葡萄糖代谢相关分子的变化特点。方法:普通级雄性巴马小型猪10只随机分为正常组及脾虚痰浊组,每组5头。采用疲劳过度联合高脂单笼饲养建立小型猪脾虚痰浊证模型。采用实时荧光定量PCR法检测心肌组织葡萄糖转运体4(glucose transporter 4,GLUT4)、磷酸果糖激酶1(Phosphofructokinase-1,PFK-1)、丙酮酸激酶(Pyruvate kinase2,PKM2)和己糖激酶(Hexokinase,HK)mRNA表达,应用Western blotting法检测心肌组织GLUT4、PFK1、PKM2和HK蛋白表达水平。结果:与正常组小型猪相比,脾虚痰浊组小型猪心肌组织GLUT4、PKM2、PFK1、HK mRNA表达水平显著下降(P0.01),GLUT4、PKM2蛋白表达水平显著下降(P0.05)。PFK1、HK蛋白表达水显著下降P0.01)。结论:脾虚痰浊证小型猪心肌存在葡萄糖代谢异常,与葡萄糖转运及代谢相关分子表达呈下降趋势。
[Abstract]:Objective: to investigate the changes of glucose metabolism molecules in myocardium of Bama miniature pigs with spleen deficiency and phlegm turbid syndrome. Methods: ten male Bama miniature pigs of common grade were randomly divided into normal group (n = 5) and spleen deficiency and phlegm turbid group (n = 5). The model of spleen deficiency and phlegm turbid syndrome of miniature pigs was established by fatigue combined with hyperlipidemia and single cage feeding. The expression of glucose transporter 4 (glucose transporter 4, glut 4, phosphofructokinase 1 (Phosphofructokinase-1,PFK-1), pyruvate kinase (Pyruvate kinase2,PKM2) and hexokinase (Hexokinase,HK) mRNA in myocardial tissue was detected by real time fluorescence quantitative PCR, and the expression of GLUT4,PFK1,PKM2 and HK protein in myocardial tissue was detected by Western blotting method. Results: compared with the normal group, the expression level of GLUT4,PKM2,PFK1,HK mRNA and GLUT4,PKM2 protein in myocardial tissue of miniature pigs with spleen deficiency and phlegm turbid group were significantly lower than those in normal group (P 0.05). The expression of PFK1 and HK protein was significantly decreased by P 0.01). Conclusion: there is abnormal glucose metabolism in the myocardium of miniature pigs with spleen deficiency and phlegm turbid syndrome, and the expression of glucose transport and metabolism related molecules is decreased.
【作者单位】： 辽宁中医药大学;辽宁中医药大学附属医院;辽宁中医药大学附属第二医院;
【基金】：国家重点基础研究发展计划(“973”计划)资助项目(2013CB531704)
【分类号】：R228;R-332
本文编号：2507009
[Abstract]:Objective: to investigate the changes of glucose metabolism molecules in myocardium of Bama miniature pigs with spleen deficiency and phlegm turbid syndrome. Methods: ten male Bama miniature pigs of common grade were randomly divided into normal group (n = 5) and spleen deficiency and phlegm turbid group (n = 5). The model of spleen deficiency and phlegm turbid syndrome of miniature pigs was established by fatigue combined with hyperlipidemia and single cage feeding. The expression of glucose transporter 4 (glucose transporter 4, glut 4, phosphofructokinase 1 (Phosphofructokinase-1,PFK-1), pyruvate kinase (Pyruvate kinase2,PKM2) and hexokinase (Hexokinase,HK) mRNA in myocardial tissue was detected by real time fluorescence quantitative PCR, and the expression of GLUT4,PFK1,PKM2 and HK protein in myocardial tissue was detected by Western blotting method. Results: compared with the normal group, the expression level of GLUT4,PKM2,PFK1,HK mRNA and GLUT4,PKM2 protein in myocardial tissue of miniature pigs with spleen deficiency and phlegm turbid group were significantly lower than those in normal group (P 0.05). The expression of PFK1 and HK protein was significantly decreased by P 0.01). Conclusion: there is abnormal glucose metabolism in the myocardium of miniature pigs with spleen deficiency and phlegm turbid syndrome, and the expression of glucose transport and metabolism related molecules is decreased.
【作者单位】： 辽宁中医药大学;辽宁中医药大学附属医院;辽宁中医药大学附属第二医院;
【基金】：国家重点基础研究发展计划(“973”计划)资助项目(2013CB531704)
【分类号】：R228;R-332
【相似文献】
相关期刊论文 前5条
1 谢慧君;;加味五苓散干预脾虚痰浊型血脂异常的临床观察[J];光明中医;2013年07期
2 冯锦;;健脾化痰祛湿方治疗高脂血症脾虚痰浊型56例[J];中国中医药科技;2012年01期
3 孔德昭;吕美君;张哲;王洋;刘悦;刘光辉;陈雪娇;杨关林;;运用德尔菲法对《冠心病稳定型心绞痛脾虚痰浊证·中医证候计分表》条目筛选的研究[J];辽宁中医杂志;2014年05期
4 毛丹丹;林士毅;苏孝共;;从脾虚痰浊论治糖调节受损患者糖、脂代谢紊乱及对血清脂联素的影响[J];中华中医药学刊;2012年04期
5 ;[J];;年期
相关硕士学位论文 前6条
1 杨茗茜;巴马小型猪脾虚痰浊模型建立与评价[D];辽宁中医药大学;2015年
2 杨庆玲;无饥饿禁食疗法治疗脾虚痰浊型肥胖型2型糖尿病临床观察[D];山东中医药大学;2016年
3 赵传鹏;健脾化痰、熄风定vN法治疗脾虚痰浊型癫vN的理论探讨及临床研究[D];山东中医药大学;2009年
4 谢慧君;加味五苓散干预脾虚痰浊型血脂异常的临床观察[D];广州中医药大学;2010年
5 杨尾莲;保肝消脂颗粒对NAFLD肝郁脾虚痰浊血瘀型患者血清抵抗素影响的临床研究[D];黑龙江中医药大学;2012年
6 张永;保肝消脂颗粒对NAFLD肝郁脾虚痰浊血瘀型患者SCD-1影响的临床研究[D];黑龙江中医药大学;2012年
，本文编号：2507009
本文链接：https://www.wllwen.com/zhongyixuelunwen/2507009.html
