自然条件下盐酸吗啡对肥须亚麻蝇幼虫发育积温和头咽骨的影响

发布时间：2018-02-01 18:48

本文关键词： 自然条件积温肥须亚麻蝇吗啡死后间隔时间头咽骨骨化面积平均光密度　出处：《第二军医大学学报》2015年11期 　论文类型：期刊论文

【摘要】：目的观察自然条件下盐酸吗啡对石家庄地区优势蝇种肥须亚麻蝇(Parasarcophaga crassipalpis)幼虫发育积温和头咽骨的影响,探讨利用尸源性蝇类头咽骨形态学指征和积温推测尸体死后间隔时间的可行性。方法自然条件下,用含吗啡的兔肉(取自用不同剂量盐酸吗啡注射后处死的兔)饲养肥须亚麻蝇幼虫,每隔12h留取幼虫标本,直至离食期。将幼虫烫死并保存。将实验期间的日均温累加计算积温。体视显微镜下分离出幼虫头咽骨并在光学显微镜下观察、拍照。利用计算机图像分析系统对头咽骨的形态学指征进行测定。结果 (1)自然条件下,盐酸吗啡一定程度上改变了肥须亚麻蝇幼虫的积温和历期。(2)自然条件下,幼虫口钩和咽骨的面积进入三龄期后维持基本恒定,而头咽骨骨化面积和平均光密度均随积温的增加而逐渐增高,直至三龄期结束。幼虫口钩、咽骨的骨化面积和平均光密度达到最大值的积温明显大于二者面积达到最大值的积温。结论自然条件下,盐酸吗啡显著改变了肥须亚麻蝇幼虫发育积温,但肥须亚麻蝇幼虫口钩和咽骨的骨化面积、平均光密度均随积温的增加而增加,呈现良好的线性趋势,并一直维持到三龄期后,它们结合积温可推测死后间隔时间。
[Abstract]:Objective to observe the effect of morphine hydrochloride on the development temperature and cephalopharynx bone of Parasarcophaga crassipalp larvae in Shijiazhuang area. To explore the feasibility of estimating postmortem interval time by morphological indication and accumulated temperature of cadaveric fly cephalopharynx. Methods under natural conditions, The larvae of flax fly were fed with morphine containing rabbit meat (taken from rabbits killed after injection with different doses of morphine hydrochloride), and the larval specimens were collected every 12 hours. The larval head and pharynx bone was separated under stereoscopic microscope and observed under optical microscope. The morphologic indications of cephalopharynx bone were determined by computer image analysis system. Results 1) under natural conditions, morphine hydrochloride changed the accumulated temperature of the larva of flax fly to a certain extent and the duration of the larva under natural conditions. The area of larval hooks and pharyngeal bones remained constant after entering the third age period, while the area of ossification of cephalopharynx bone and the average optical density increased gradually with the increase of accumulated temperature until the end of the third age. The cumulative temperature of ossification area and average optical density of pharyngeal bone was significantly higher than that of the maximum area of both. Conclusion under natural conditions, morphine hydrochloride can significantly change the accumulated temperature of larval development of Flax fly. However, the ossification area of larval hooks and pharyngeal bones of Flina melanogaster larva increased with the increase of accumulated temperature, and showed a good linear trend, and they could be combined with accumulated temperature to predict the postmortem interval time.
【作者单位】：河北中医学院中西医结合学院;河北中医学院实验中心;
【分类号】：D919
【正文快照】： 肥须亚麻蝇(Parasarcophaga crassipalpis)在我国分布广泛,成蝇较早飞临腐败尸体,幼虫尸食性,具有重要的法医学意义。探讨毒物对尸源性昆虫及其幼虫发育历期的影响,进而推测死后间隔时间(postmorteminterval,PMI),是法医昆虫毒理学的一个重要研究方向[1-2]。头咽骨是蝇幼虫头

【参考文献】