大鼠皮肤切创愈合过程中E-选择素及ICAM-1表达及法医学意义

发布时间：2018-02-05 17:06

本文关键词： E-选择素细胞间粘附分子- 皮肤切伤损伤时间推断免疫组织化学　出处：《重庆医科大学学报》2009年07期 　论文类型：期刊论文

【摘要】：目的:探讨大鼠皮肤切创愈合过程中E-选择素(E-selectin)及细胞间粘附分子-1(Intercellular adhesion molecule-1,ICAM-1)表达变化规律及法医学意义。方法:建立模型后,应用免疫组化方法,观察大鼠生前皮肤切创后不同时间点E-选择素及ICAM-1的表达变化,并用图像分析系统对图像分析。结果:正常组无E-选择素表达,切创后1hE-选择素在损伤周围血管内皮细胞有阳性表达,伤后1d达峰值,以后开始下降,持续至伤后7d,且E-选择素随时间呈规律性变化。ICAM-1最早在伤后1h,最迟在伤后3d在表皮层中呈阳性表达。此外,ICAM-1还在炎症细胞、成纤维细胞及损伤周围血管内皮细胞有阳性表达,且随损伤时间呈规律性变化。E-选择素及ICAM-1在死后伤中均为阴性表达。结论:E-选择素及ICAM-1可作为法医学损伤时间的参考指标。
[Abstract]:Objective: to investigate Eselectin E-selectin (E-selectin) and intercellular adhesion molecule-1 (ICAM-1) during wound healing of rat skin. Intercellular adhesion molecule-1. Methods: after the establishment of the model, the immunohistochemical method was used. The changes of E- selectin and ICAM-1 expression at different time points after skin incision were observed and analyzed by image analysis system. Results: there was no Eselectin expression in normal group. The expression of E- selectin was positive in vascular endothelial cells around injury at 1 hour after injury, reached its peak at 1 day after injury, then began to decrease, and continued to decrease until 7 days after injury. The expression of E- selectin was regular with time. ICAM-1 was positive in the epidermis at the earliest 1 hour after injury and 3 days after injury. In addition, ICAM-1 was also expressed in inflammatory cells. The positive expression of fibroblasts and vascular endothelial cells around injury was observed. The expression of E- selectin and ICAM-1 were negative in postmortem injury. Conclusion: E- selectin and ICAM-1 can be used as reference indexes of forensic injury time.
【作者单位】：重庆医科大学基础医学院法医教研室;
【分类号】：D919

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