重庆土家族群体15个STR基因座的遗传多态性

发布时间：2018-03-09 21:58

本文选题：土家族　切入点：短串联重复序列　出处：《重庆医科大学学报》2009年11期 　论文类型：期刊论文

【摘要】：目的:研究重庆土家族人群无关个体的15个STR基因座(CSF1PO、D13S317、D16S539、D18S51、D19S433、D21S11、D2S1338、D3S1358、D5S818、D7S820、D8S1179、FGA、TH01、TPOX、vWA)的遗传多态性。方法:应用AmpFlSTR Identifiler荧光标记复合扩增系统对115名重庆土家族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI310遗传分析仪对扩增产物进行检测,用GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果:15个STR基因座均符合Hardy-Weinberg平衡,杂合度在0.634 8～0.887 0之间,累积匹配概率和累积非父排除率分别为3.12×10-17和0.999 997 688 3。结论:15个STR基因座的累积匹配概率和累积非父排除率较高,适用于法医学亲子鉴定和个人识别。
[Abstract]:Objective: to study the genetic polymorphisms of 15 STR loci in unrelated individuals of Tujia nationality in Chongqing. Methods: the multiplex amplification of 15 STR loci was carried out in 15 unrelated individuals of Chongqing Tujia nationality by using the AmpFlSTR Identifiler fluorescent marker system to amplify 15 STR loci of D19S433D2S1338, D3S1338, D7S820D8S1179FGAFGAT01TPOXVWA. methods: a AmpFlSTR Identifiler fluorescent marker system was used to amplify 15 DNA loci of blood samples from 115 unrelated individuals of Chongqing Tujia nationality. The amplified products were detected by ABI310 genetic analyzer and genotypes were carried out by GeneScann GenoTyper software. The population genetic parameters of 15 STR loci were calculated statistically. Results: all the 15 STR loci accord with Hardy-Weinberg equilibrium, and the heterozygosity is between 0.634 8 and 0.887 0. The cumulative matching probability and cumulative non-paternal exclusion rate were 3.12 脳 10 ~ (-17) and 0.999 997 688.3 respectively. Conclusion: the cumulative matching probability and cumulative non-paternal exclusion rate of 15 STR loci are higher, which is suitable for forensic paternity testing and personal identification.
【作者单位】：重庆医科大学基础医学院法医学教研室;
【分类号】：D919.4;R394

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