S100A4蛋白和P53蛋白在肥厚型心肌病导致的心肌纤维化中的表达及意义

发布时间：2018-03-09 22:43

本文选题：S100A4　切入点：P53　出处：《青岛大学》2016年硕士论文　论文类型：学位论文

【摘要】：【目的】心肌纤维化是肥厚型心肌病的典型病理改变之一,而S100A4、P53蛋白与心肌纤维化的发生联系密切。本实验旨在研究肥厚型心肌病患者者心肌细胞中S100A4、P53蛋白的表达,探讨其与肥厚型心肌病心肌纤维化的关系。【方法】选择于2013年1月-2014年6月就诊于北京阜外心血管医院的肥厚性心肌病手术患者10例作为HCM组,另选择10例于青岛地区交通事故及高坠致死者心肌组织为健康对照组,运用苦味酸天狼猩红染色法比较两组的胶原容积分数、原位杂交方法检测S100A4和P53 m RNA表达情况以及运用免疫组化法比较2组心肌组织内S100A4、P53阳性表达的变化;运用原位杂交方法检测S100A4、P53 m RNA表达情况。利用Image-Pro Plus 6.0等图像分析软件进行染色结果分析。统计结果进行t检验,以P0.01表示为存在显著性差异。【结果】HCM组心肌间质胶原容积分数高于对照组(t=4.105,P0.01),HCM组心肌细胞S100A4、P53蛋白表达明显高于对照组(t=7.628,P0.01)以及二者m RNA阳性表达均高于对照组(t=3.464,P0.01)差异均有高度统计学意义。【结论】S100A4、P53蛋白的过度表达可能在HCM心肌纤维化过程中发挥重要作用,可能为肥厚型心肌病心肌纤维化的治疗提供新的方向。
[Abstract]:[objective] Myocardial fibrosis is one of the typical pathological changes in hypertrophic cardiomyopathy. To investigate the relationship between hypertrophic cardiomyopathy and myocardial fibrosis. [methods] Ten patients with hypertrophic cardiomyopathy treated in Fuwei Cardiovascular Hospital from January 2013 to June 2014 were selected as HCM group. In addition, 10 cases of myocardial tissue in Qingdao area were selected as the healthy control group. The collagen volume fraction of the two groups was compared by picric acid sirius red staining method. In situ hybridization was used to detect the expression of S100A4 and p53 m RNA, and immunohistochemical method was used to compare the expression of S100A4 and p53 in myocardium. In situ hybridization method was used to detect the expression of p53 m RNA in S100A4, and the staining results were analyzed by Image-Pro Plus 6.0 and other image analysis softwares. The statistical results were analyzed by t test. [results] the myocardial interstitial collagen volume fraction in the HCM group was significantly higher than that in the control group (t 4.105 and P 0.01), and the expression of S100A4P 53 protein in the HCM group was significantly higher than that in the control group (P 0.01), and the positive expression of m RNA was higher than that in the control group (t 3.464P 0.01). [conclusion] the overexpression of S100A4 protein may play an important role in the process of myocardial fibrosis in HCM. It may provide a new direction for the treatment of hypertrophic cardiomyopathy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：D919

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