利用Identifiler分型系统推断同胞关系

发布时间：2018-03-24 12:01

  本文选题：法医物证学　切入点：常染色体　出处：《中国法医学杂志》2009年06期

【摘要】：目的探讨自主开发的同胞关系鉴定自动分析软件(ASI)对Identifiler分型系统进行同胞关系鉴定的可行性。方法应用本课题组所开发的软件ASI,对151对同胞及31 224对人工模拟无关个体进行Identifiler系统的15个常染色体STR基因座分型进行分析,计算亲权指数(PI)、同胞关系概率(WFS)和等位基因匹配情况,所获数据进行统计分析,自动计算排序。结果当WFS大于99.999%时,同胞个体占39.07%,无关个体占0%,两组具有显著差异,可以推断两个体同胞关系。当WFS介于1%~99.999%范围内,同胞个体和无关个体有部分重叠,同胞个体占60.93%,无关个体占21.3%,两者具有一定差异,可以通过增加检测STR基因座,再结合案情加作Y-STR、m tDNA检测,以推断两个体是否具有同胞关系。当WFS小于1%时,同胞个体占0%,无关个体占78.7%,可以推断两个体不具有同胞关系。个体间等位基因匹配结果表明:在检测Identifiler体系15个STR基因座时,当两个体常染色体STR基因座的全相同数目≥5时,或全不同数目≤1时,提示为同胞关系;当两个体全不同数目≥6时,或全相同数目≤1时,提示为无关个体,以此作为预测有无同胞关系的界值。结论Identifiler系统及同胞关系鉴定自动分析软件ASI可用于推断同胞关系。
[Abstract]:Objective to explore the feasibility of sibling relationship identification in Identifiler typing system developed by our own developed automatic sibling relationship analysis software (Identifiler). Methods using the software developed by our team, 151 pairs of sibling and 31 224 pairs of artificial simulation were tested without artificial simulation. The 15 autosomal STR loci of the Identifiler system were analyzed. The paternity index, sibling probability and allele matching were calculated. The obtained data were statistically analyzed and sorted automatically. Results when the WFS was greater than 99.999, the sibling individuals accounted for 39.07 and unrelated individuals took up 00.There was a significant difference between the two groups. It can be inferred that when WFS is within the range of 99.999%, there is a partial overlap between the sibling individual and the unrelated individual, the sibling individual occupies 60.9333um and the unrelated individual occupies 21.3B. there is a certain difference between the two. We can detect the STR locus by increasing the detection of the STR locus. Combined with the case, Y-STRM tDNA test is used to infer whether the two bodies have sibling relationship. When WFS is less than 1, It can be inferred that there is no sibling relationship between the two bodies. The results of allelic gene matching between individuals indicate that when 15 STR loci in the Identifiler system are detected, when the number of STR loci of two autosomes is equal to or greater than 5, the number of STR loci of two autosomes is more than 5. When the number of two bodies is equal to or less than 6, or the same number is 鈮，

本文编号：1658201