死亡大鼠看家基因mRNA时序性降解的组织差异性研究

发布时间：2018-04-04 07:45

本文选题：法医病理学　切入点：死亡时间推断　出处：《中国法医学杂志》2009年03期

【摘要】：目的研究死亡大鼠看家基因mRNA时序性降解的组织差异性,评价其用于死亡时间推断的价值。方法SD大鼠20只分为死后0、1、3、5、7d共5组,脊椎脱臼法处死大鼠,提取大鼠心、肝、脾、肺、肾、脑组织,在相应时间段提取RNA,应用一步法RT-PCR技术检测各组织中看家基因GAPDH mRNA和β-actin mRNA的水平,Vilber凝胶图像分析系统测定扩增产物IOD值,SPSS 10.0统计软件对数据进行方差分析。结果GAPDH mRNA、β-actin mRNA扩增产物相对灰度与积分光密度值随死后经过时间的延长而逐渐减小,且与死亡时间显著相关,大鼠脾脏和脑组织GAPDH mRNA和β-actin mRNA在死后5d内可检出,心脏和肾脏在死后3d内可检出,而肝脏和肺脏GAPDH mRNA和β-actin mRNA降解较快,仅在死后1d内可检出。结论脑组织和脾脏中mRNA稳定性较好,适用于PMI特别是晚期PMI的推断。除环境温度外,环境湿度也是死亡时间推断中的重要影响因素。
[Abstract]:Objective to study the tissue difference of mRNA timing degradation of dead rat housekeeping gene and evaluate its value in estimating death time.Methods Twenty Sprague-Dawley rats were divided into 5 groups for 7 days after death. The rats were killed by spinal dislocations and the heart, liver, spleen, lung, kidney and brain were extracted.GAPDH mRNA and 尾 -actin mRNA were detected by one-step RT-PCR technique in the corresponding time period. The IOD value of the amplification product was analyzed by using SPSS10.0 statistical software to analyze the variance of the data. The results were as follows: (1) GAPDH mRNA and 尾 -actin mRNA (尾 -actin mRNA) were detected by SPSS10.0.Results the relative grayscale and integrated optical density of GAPDH mRNAand 尾 -actin mRNA products decreased with the prolongation of postmortem time, and were significantly correlated with the death time. GAPDH mRNA and 尾 -actin mRNA in spleen and brain tissue of rats could be detected within 5 days after death.The heart and kidney could be detected within 3 days after death, but the GAPDH mRNA and 尾 -actin mRNA in liver and lung were degraded more rapidly, and only within 1 day after death.Conclusion the stability of mRNA in brain tissue and spleen is good, which is suitable for PMI, especially for late PMI.In addition to ambient temperature, environmental humidity is also an important factor in the estimation of death time.
【作者单位】：山西医科大学法医学院;太原市公安局尖草坪分局;广州市公安局交警支队;
【基金】：山西省高校科技研究开发项目(20051224) 山西医科大学博士启动基金资助(03200716)
【分类号】：D919

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