超高效液相色谱-高分辨质谱联用分析新型毒品5F-AMB的体外肝微粒体代谢物及代谢途径

发布时间：2018-04-13 06:32

  本文选题：N-(-甲氧基羰基--甲基丙基)--(-氟戊基)吲唑--甲酰胺 + 合成大麻素　； 参考：《药学学报》2017年11期

【摘要】：本文研究新型毒品5F-AMB在体外肝微粒体模型中的代谢物。在体外人肝微粒体孵育模型中加入5F-AMB以模拟在人体的代谢过程,并利用超高效液相色谱高分辨质谱联用技术(UPLC-HR-MS)分析代谢发生位点及代谢途径。结果显示,5F-AMB在人肝微粒体模型中共产生9种代谢物,其主要代谢途径为酯键水解、酯键水解合并脱氟氧化、酯键水解合并戊烷基链羟化和酯键水解合并吲唑环羟化。以上结果表明该方法简便快捷,推荐5F-AMB的酯键水解、酯键水解合并脱氟氧化、酯键水解合并戊烷基链羟化和酯键水解合并吲唑环羟化代谢物为合适的潜在尿液标志物。
[Abstract]:The metabolites of new drug 5F-AMB in vitro liver microsomal model were studied.5F-AMB was added to human liver microsomal incubation model in vitro to simulate the metabolic process in human body. UPLC-HR-MS was used to analyze the metabolic sites and pathways.The results showed that 9 metabolites were produced in human liver microsomal model. The main metabolic pathways were ester bond hydrolysis, ester bond hydrolysis combined with defluorination, ester bond hydrolysis combined with pentane chain hydroxylation and ester bond hydrolysis combined with indazole hydroxylation.The results show that this method is simple and fast. It is recommended that the hydrolysis of ester bond, hydrolysis of ester bond combined with defluorination, hydrolysis of ester bond combined with hydroxylation of pentane chain and hydrolysis of ester bond combined with indazole cyclohydroxylation metabolite are suitable potential urine markers.
【作者单位】： 公安部禁毒情报技术中心;
【基金】：国家重点研究与发展计划资助项目(2016YFC0800903)
【分类号】：D919.4
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本文编号：1743308