超高效液相色谱-串联质谱法检测血液斑痕中磺酰脲类降糖药

发布时间：2018-04-14 21:28

本文选题：磺酰脲类降糖药 + 血液斑痕　；参考：《分析试验室》2017年12期

【摘要】：建立了血液斑痕中格列本脲,格列美脲,格列吡嗪的液相色谱-质谱检验法。选用ACQUITY UPLC HSS C18(2.1×50 mm,1.8μm)色谱柱,使用0.1%甲酸水-乙腈体系作为流动相进行梯度洗脱。采用ESI+电喷雾离子源,多反应监测扫描模式(MRM)检测3种磺酰脲类降糖药。在最优条件下,磺酰脲类降糖药在1.25~250 ng/patch(swab)范围内有良好线性关系,保留时间依次为2.90,3.00,2.34 min。检出限为0.1 ng/mL。渗透性客体上药物的回收率随着浓度的增大而增大,非渗透性客体上药物的回收率随着浓度的增大而减小。日内与日间精密度均小于15%,基质效应在0.85~1.15之间。可用于血液斑痕中格列本脲,格列美脲,格列吡嗪的快速筛查。
[Abstract]:A liquid chromatography-mass spectrometry method was developed for the determination of glibenclamide, glimepiride and glipizide in blood stains.A ACQUITY UPLC HSS C1818 (2. 1 脳 50 mm ~ (-1) 渭 m) column was used. The mobile phase consisted of 0.1% formic acid water and acetonitrile as the mobile phase for gradient elution.Three sulfonylurea hypoglycemic drugs were detected by ESI electrospray ion source and multiple reaction monitoring scanning mode (MRM).Under the optimum conditions, the sulfonylurea antidiabetic drugs had a good linear relationship in the range of 1.25 ~ 250ng / r patchswabs, and the retention time was 2.90 ~ 3.00 ~ 2.34 min.The detection limit was 0.1 ng / mL.The recovery rate of drugs on osmotic guest increased with the increase of concentration, and decreased with the increase of concentration in non-permeable guest.Both intra-day and inter-day precision were less than 15. The matrix effect was between 0.85 and 1.15.It can be used for rapid screening of glibenclamide, glimepiride and glipizide in blood stains.
【作者单位】：中国人民公安大学;公安部物证鉴定中心;
【基金】：国家重点研发计划(2017YFC0803504) 公安部技术研究计划(2015JSYJC49)项目资助
【分类号】：D918.93;O657.63

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