唾液中苯丙胺类毒品的毛细管区带电泳在线富集检测

发布时间：2018-04-15 12:56

本文选题：毛细管区带电泳 + 场放大样品堆积　；参考：《分析测试学报》2008年05期

【摘要】：采用毛细管区带电泳(CZE)中的场放大样品堆积(FASS)技术对唾液中的苯丙胺类毒品进行了检测。采用含30%(体积分数)甲醇的100 mmol/L磷酸盐(pH 3.0)为分离缓冲液,用水溶解样品,利用缓冲体系与样品溶液体系电导率的差异,在毛细管中浓缩样品组分,对苯丙胺(AM)、甲基苯丙胺(MAM)、3,4-(亚甲二氧基)苯丙胺(MDA)、3,4-(亚甲二氧基)甲基苯丙胺(MDMA)4种毒品进行了分离和定量测定。采用利多卡因为内标,对添加上述4种毒品的唾液进行液液提取并进行FASS-CZE检测,可检测到的上述毒品质量浓度为0.002 mg/L,与常规毛细管区带电泳比较,灵敏度提高上千倍;相对标准偏差在1.4%~7.7%之间,回收率在74%~108%。该方法可用于生物检材中苯丙胺类毒品的检测。
[Abstract]:The field amplified sample stacking (FASS) technique in capillary zone electrophoresis (CZE) was used to detect amphetamines in saliva.In this paper, 100 mmol/L phosphate solution containing 30% methanol (volume fraction) was used as the separation buffer, the sample was dissolved with water, and the sample components were concentrated in the capillary by using the difference of conductivity between the buffer system and the sample solution system.Four kinds of drugs, Amphetamine (AMN), methamphetamine (MAM) (methylene dioxy) (methylene dioxy), methamphetamine (methylene dioxy), methamphetamine (methamphetamine) and methamphetamine (MDMA), were separated and quantitatively determined.Using Lidoca as internal standard, the saliva of the above four drugs was extracted and detected by FASS-CZE. The mass concentration of the above drugs was 0.002 mg / L, which was thousands of times higher than that of conventional capillary zone electrophoresis.The relative standard deviation was between 1.4% and 7.7%, and the recovery was 74%.The method can be used for the detection of amphetamines in biological samples.
【作者单位】：中国人民公安大学刑事科学技术系中国人民公安大学刑事科学技术系
【基金】：北京教育委员会共建项目建设计划(SYS1004100436)
【分类号】：D918.2

【共引文献】