液相色谱-串联质谱法测定毛发中的司坦唑醇

发布时间：2018-04-19 07:45

本文选题：液相色谱-串联质谱 + 司坦唑醇　；参考：《色谱》2008年04期

【摘要】：采用液相色谱-串联质谱(LC-MS/MS)建立了毛发中司坦唑醇的分析方法,并将其应用于单次给药动物实验中的动物毛样品分析。将10mg样品碱水解后加入戊烷提取,然后进行LC-MS/MS分析,采用正离子电喷雾电离、多反应监测模式测定,方法的最低定量限为25pg/mg。剃去豚鼠背部中央的毛,以60mg/kg的剂量于豚鼠腹腔注射司坦唑醇,然后隔天在同一部位剃取其毛,两周内该豚鼠毛中均能检出司坦唑醇;给药后豚鼠毛中司坦唑醇的含量在第1周内保持稳定,在给药后第10天达到峰值。所建立的方法毛发取样量少,特异性强,灵敏度高,适用于毛发中司坦唑醇的分析。
[Abstract]:A method for the determination of stetazolol in hair was established by liquid chromatography-tandem mass spectrometry (LC-MS / MS). The method was applied to the analysis of animal hair samples in single dose animal experiment.The 10mg sample was extracted with pentane after alkaline hydrolysis, and then analyzed by LC-MS/MS. The method was determined by positive ion electrospray ionization and multi-reaction monitoring mode. The minimum quantitative limit of the method was 25pg / mg.After shaving the hair in the center of the back of the guinea pig, the guinea pig was injected intraperitoneally with stetazolol at the dose of 60mg/kg, and the hair was shaved at the same place the next day. Within two weeks, the hair of the guinea pig could be detected.The content of stetazolol in guinea-pig hair remained stable in the first week and reached its peak on the 10th day after administration.The method is suitable for the analysis of stetazolol in hair because of its low sampling quantity, high specificity and high sensitivity.
【作者单位】：司法部司法鉴定科学技术研究所上海市法医学重点实验室;
【基金】：国家自然科学基金项目(编号:20475037) 上海自然科学基金项目(编号:07ZR14114)资助
【分类号】：D919

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