华东地区汉族人群7个Y-STR基因座遗传多态性的研究

发布时间：2018-05-05 19:30

本文选题：短串连重复(STR) + Y染色体　；参考：《苏州大学》2009年硕士论文

【摘要】： 目的:调查5个新的Y-STR基因座DYS708、DYS712、DYS713、DYS714、DYS715及经验证多态性好的2个DYS576、DYS709在华东地区汉族人群中遗传多态性,评估其在法医学中的应用价值;建立DYS708、DYS713、DYS715复合扩增体系。方法:随机筛选108名华东地区汉族男性个体,Chelex-100方法提取样本DNA,利用PCR扩增、聚丙烯酰胺凝胶电泳、硝酸银染色进行分型检测DYS576、DYS708、DYS709、DYS712、DYS713、DYS714、DYS715基因座的遗传多态性。对7个基因座等位基因测序,构建等位基因标准物,按照ISFG原则命名等位基因。结果:DYS708、DYS712、DYS713、DYS714、DYS715、DYS576、DYS709在该人群中分别检出了7、13、7、10、7、6和8个等位基因,其基因多样性分别为0.7242、0.9115、0.8041、0.8492、0.8121、0.7351和0.8202,这7个Y-STR基因座的单倍型基因多样性是0.99999851。测序结果显示:在所选的Y-STR基因座中DYS708是复合重复序列,其他是简单复合序列, DYS708、DYS712、DYS713、DYS714、DYS715、DYS576、DYS709的串联重复单位分别为AGAT/AGCA、ATCT、TCTT、CTTTT、AGAT、AAAG和CTTT。建立了银染复合扩增检测3个Y-STR基因座的分型方法,其分析结果正确,具有良好的稳定性和较好的灵敏性。结论:这7个Y-STR基因座DYS576、DYS708、DYS709、DYS712、DYS713、DYS714、DYS715基因座具有较高的遗传多态性,在法医学及人类遗传学方面具有较高应用价值。这3个Y-STR复合扩增体系在建立Y染色体STR数据库、研究群体遗传和进行法医学鉴定有重要意义。
[Abstract]:Objective: to investigate the genetic polymorphisms of five new Y-STR loci, DYS708, DYS712, DYS713, DYS714, DYS715 and 2 DYS576DYS709, and to evaluate their application value in forensic medicine, and to establish a DYS708DYS713DYS715 multiplex amplification system. Methods: the DNA samples were extracted by Chelex-100 method from 108 Han males in East China. The genetic polymorphisms of DYS576 DYS708 / DYS709 / DYS712DYS713 / DYS714DYS715 locus were detected by PCR amplification, polyacrylamide gel electrophoresis and silver nitrate staining. The alleles of 7 loci were sequenced and the allele standard was constructed. The alleles were named according to ISFG principle. Results DYS708 / DYS712DYS713 / DYS714DYS715 / DYS576DYS709 alleles were detected in this population, respectively. The genetic diversity of the 7 Y-STR loci was 0. 999998551 and 0. 99995851respectively, and the genetic diversity was 0. 72420.91150.8041ng0.84920.8121a 0.7351 and 0. 82022. The genetic diversity of the 7 Y-STR loci was 0. 99999851. the genetic diversity of these alleles was 0. 99999851. the genetic diversity of DYS708, DYS712, DYS713, DYS713 and DYS713 was 0. The sequencing results showed that DYS708 was a complex repeat sequence in the selected Y-STR locus, while the other was a simple complex sequence. The tandem repeat units of DYS708 / DYS712 / DYS713 / DYS715 / DYS576 / DYS709 were AGATR / AGCAATATCTT TCTTTTT AGATA AG and CTT T, respectively. A silver staining multiplex amplification method for the detection of three Y-STR loci was established. The results were correct and had good stability and sensitivity. Conclusion: these seven Y-STR loci DYS576DYS708DYS709 / DYS712DYS713DYS714DYS715 have high genetic polymorphism and have high application value in forensic medicine and human genetics. These three Y-STR multiplex amplification systems are of great significance in the establishment of Y chromosome STR database, the study of population inheritance and forensic identification.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：D919

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