氯硝安定在生物体内的代谢

发布时间：2018-05-13 23:09

本文选题：法医毒物分析 + 氯硝安定　；参考：《中国法医学杂志》2009年05期

【摘要】：目的推测氯硝安定在生物体内的主要代谢方式,检测其代谢产物。方法取5只Wistar大鼠,连续3d给每只大鼠灌胃氯硝安定(剂量分别为1、2、2mg),收集灌胃后24h内尿液;给3只大鼠各灌胃2mg氯硝安定,2h后处死,取血液和肝组织等检材;收集3名口服5mg氯硝安定的志愿者24h内尿液。分别对不同检材进行处理后,气相色谱/质谱联用(GC/MS)检测。结果在大鼠阳性尿液和志愿者阳性尿液中,均检出7-乙酰氨基氯硝安定和7-氨基氯硝基安定;在大鼠血液中,主要检出氯硝安定和7-氨基氯硝基安定;在大鼠肝组织中,主要检出7-乙酰氨基氯硝安定,还有少量7-氨基氯硝安定和氯硝安定。结论氯硝安定进入生物体后,其7位硝基被还原为氨基,氨基接着被乙酰化,形成7-氨基氯硝基安定和7-乙酰氨基氯硝安定代谢物,其中7-乙酰氨基氯硝安定为主要代谢物。
[Abstract]:Objective to speculate on the main metabolic patterns of clonazepam in the organism. Methods 5 Wistar rats were taken, and 3D was given to each rat by gavage of clonazepam (1,2,2mg, respectively), and the urine was collected in 24h after gavage. 3 rats were given 2mg clonazepam and 2h after 2h, and the blood and liver tissues were taken and 3 mouth were collected. The urine of 5mg clonazepam was taken in the urine of 24h. The results were detected by gas chromatography / mass spectrometry (GC/MS). The results showed that 7- acetaminophen and 7- aminochlorinazepam were detected in the positive urine of the rats and the positive urine of the volunteers. In the blood of the rats, the clonazepam and the 7- aminochlorinyl nitro group were detected. Diazepam, 7- acetaminophen, and a small amount of 7- aminclonazepam and clonazepam were found in the rat liver. Conclusion the 7 nitro groups were reduced to amino groups and then acetaminated to form 7- amino chloro nitroazazazepam and 7- acetaminophen tranidazepam metabolites, in which the 7- acetyl amino group was formed. Clonazepam is the main metabolite.

【作者单位】：广西壮族自治区公安厅物证鉴定中心;广西大学化学化工学院;广西壮族自治区公安厅科技处;
【基金】：公安部应用创新计划资助项目(2007YYCXGXST081)
【分类号】：D919

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