SPE-HPLC法分析大鼠血浆中灭多威

发布时间：2018-05-16 21:13

  本文选题：法医毒物分析 + 灭多威　； 参考：《中国法医学杂志》2009年06期

【摘要】：目的建立以固相萃取-高效液相色谱(SPE-HPLC)分析大鼠血浆中灭多威的方法。方法以空白大鼠血浆添加灭多威标准品及内标物,以SPE法对样品进行前处理,采用HPLC进行分析,考察仪器测试条件、特异性、回收率、灵敏度、精密度、准确度、线性关系、稳定性,并对方法进行优化。结果采用本文方法测得灭多威在血浆中的线性范围0.1~20μg/mL(r=0.9993,P0.001),血浆中的检测限0.03μg/mL(S/N≥3),日内、日间精密度的CV值分别在8.33%与11.11%以内,日内、日间准确度值分别在90%与120%之间,回收率值在88%±4.4%以上。结论所建方法实用、便捷,可对血浆中的灭多威进行定性定量地快速测定。
[Abstract]:Objective to establish a SPE-HPLC method for the determination of methomyl in rat plasma. Methods the samples were pretreated with SPE method and analyzed by HPLC with blank rat plasma, and the specificity, recovery, sensitivity, precision, accuracy and linear relationship were investigated. Stability and optimization of the method. Results the linear range of methomyl in plasma was 0.1 ~ 20 渭 g 路mL ~ (-1) ~ 0.9993 渭 g / m ~ (-1), the detection limit was 0.03 渭 g/mL(S/N 鈮，

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