组织中三甲胺-氮浓度变化与晚期PMI关系初探

发布时间：2018-06-12 18:34

本文选题：法医病理学 + 死亡时间　；参考：《中国法医学杂志》2009年04期

【摘要】：目的探索死后尸体组织中三甲胺-氮(TMA-N)浓度变化规律及其与晚期死亡时间的关系。方法健康SD大鼠66只,颈椎脱臼法处死,随机分成11组,分别于死后即刻、1、2、3、4、5、6、7、8、9、10d取大鼠肌肉、肝脏和肾脏组织,用分光光度法检测TMA-N浓度,所得数据用方差分析比较组间差异性,并进行回归方程拟合。结果3种组织中TMA-N含量随死后时间延长而增加,肌肉于死后7d、肝脏和肾脏于死后8d达到高峰,之后有所下降,并均于死后10d再次升高,肝、肾组织之间TMA-N含量比较无统计学差异性。死后2~7d内,PM I与肌肉TMA-N浓度变化拟合度最高(R2=0.969);死后3~8d内,与肝肾中TMA-N浓度变化拟合度最高(R2=0.953)。结论死后组织中TMA-N含量变化与PM I有相关性,可望为晚期PM I推断提供参考,但肌肉与肝肾TMA-N含量变化规律存在一定差异,需选择最适时段以提高PM I推断的准确性。
[Abstract]:Objective to explore the changes of TMA-N concentration in postmortem cadavers and its relationship with late death time. Methods 66 healthy Sprague-Dawley rats were killed by cervical dislocated method, and were randomly divided into 11 groups. The muscle, liver and kidney tissues of the rats were collected at 10 days after death. The concentration of TMA-N was detected by spectrophotometry. The data were compared by ANOVA between the two groups. The regression equation was fitted. Results the content of TMA-N in the three tissues increased with the prolongation of postmortem time. The liver and kidney reached a peak at 7 days after death, and then decreased, and then increased again at 10 days after death, liver. There was no significant difference in TMA-N content between renal tissues. Within 2 days after death, the best fit between PM I and TMA-N concentration in muscle was 0.969G, and the highest fit between PM I and TMA-N concentration in liver and kidney was 0.953% within 3 days after death. Conclusion the changes of TMA-N content in postmortem tissues are correlated with PM I, which is expected to provide a reference for the late stage of PM I inference, but there are some differences between the muscle and liver and kidney TMA-N contents, so it is necessary to select the best time to improve the accuracy of PM I inference.
【作者单位】：华中科技大学同济医学院法医学系;安阳市公安局;中国政法大学证据科学教育部重点实验室;
【基金】：湖北省卫生厅科研基金资助项目(JX2B02)
【分类号】：D919

【参考文献】