创伤性脑损伤大鼠水通道蛋白4表达变化及法医学意义

发布时间：2018-07-10 12:23

本文选题：法医病理学 + 颅脑损伤　；参考：《中国法医学杂志》2008年06期

【摘要】：目的观察大鼠颅脑损伤后不同时间内水通道蛋白4(AQP4)mRNA表达的变化,探讨AQP4在脑损伤经过时间推断中的意义。方法利用液压冲击法制作不同程度大鼠颅脑损伤模型,在伤后不同时间(0.5、2、6、12、24、48、72h),应用RT-PCR法检测脑组织AQP4 mRNA表达,同时以非损伤组做对照。结果不同程度颅脑损伤后0.5h脑组织AQP4 mRNA表达均开始上调(P0.01),6、12h依次增高,24h达到高峰,差异均有统计学意义(P0.01),72h时仍维持较高水平(P0.01);0.5h时轻度、中度、重度脑损伤时AQP4 mRNA的表达差异两两之间无统计学意义(P0.05),2、6、12、24、48、72h轻度、中度、重度脑损伤时AQP4 mRNA的表达差异两两之间有统计学意义(P0.01)。结论颅脑损伤后AQP4 mRNA呈现出时序性变化,其变化规律可望成为法医学推断早期脑损伤时间的指标之一。
[Abstract]:Objective to observe the changes of aquaporin-4 (AQP4) mRNA expression in rats with craniocerebral injury at different time points and to explore the significance of AQP4 in brain injury time inference. Methods the rat brain injury model with different degree was made by hydraulic shock method. The expression of AQP4 mRNA in brain tissue was detected by RT-PCR at different time after injury (0.5U / min) and non-injury group (n = 72). The expression of AQP4 mRNA in brain tissue was detected by RT-PCR. Results the expression of AQP4 mRNA in brain tissue began to up-regulate at 0.5 h after different degree of brain injury (P0.01) and reached its peak at 6h and 12h. The difference was statistically significant (P0.01) and maintained a high level (P0.01) at 0.5 h (P0.01). There was no significant difference in AQP4 mRNA expression between two groups in severe brain injury (P0.05). There was significant difference in AQP4 mRNA expression between two groups (P0.01). Conclusion AQP4 mRNA changes in time series after craniocerebral injury, which may be one of the predictors of early brain injury in forensic medicine.
【作者单位】：山西医科大学法医学院;山西医科大学第一医院;大同市第三人民医院;山西省高级人民法院司法技术鉴定中心;
【分类号】：D919

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