47个SNPs复合检测方法的建立及其法医学应用

发布时间：2018-07-12 14:19

本文选题：法医物证学 + 单碱基延伸(SBE)　；参考：《中国法医学杂志》2009年04期

【摘要】：目的建立47-p lex SNPs复合检测方法,评价其在法医学中的应用价值。方法筛选46个常染色体SNPs和1个Y-SNPs,使用2个检测体系分别对47个SNPs进行单管内复合PCR扩增,采用荧光标记单碱基延伸法和毛细管电泳检测技术进行分型检测;并用建立的方法对260份广东地区无关个体血样进行47个SNPs分型。结果建立的47-p lex SNPs的复合检测体系灵敏度高,种属特异性好;260名个体所有SNPs均能准确分型,群体内基因型频率分布均符合Hardy-W e inberg平衡,累积个人识别率大于0.999 9,累积非父排除率为0.999 82,累积偶合率为6.24×10-20。结论本文47-p lex SNPs复合检测方法能同时对47个SNPs进行快速、准确的检测,在法医学个体识别鉴定中具有良好的应用前景。
[Abstract]:Objective to establish a 47-p lex SNPs complex detection method and evaluate its application value in forensic medicine. Methods Forty-six autosomal SNPs and one Y-SNPswere screened. 47 SNPs were amplified by single-tube multiplex PCR using two detection systems. The established method was used for 47 SNPs typing of 260 unrelated individual blood samples in Guangdong area. Results the multiplex detection system of 47-p lex SNPs was highly sensitive and could be accurately typed in 260 individuals with good species-specificity. The frequency distribution of genotypes within the population was in accordance with the Hardy-W inberg equilibrium. The cumulative personal identification rate is greater than 0.999, the cumulative non-paternal exclusion rate is 0.999 82, and the cumulative coupling rate is 6.24 脳 10 ~ (-20). Conclusion 47-p lex SNPs compound detection method can detect 47 SNPs quickly and accurately at the same time, and has a good application prospect in forensic individual identification.
【作者单位】：广州市刑事科学技术研究所;中山大学中山医学院法医学系;广州市公安局南沙分局;
【基金】：国家十一.五科技支撑计划项目(2006BAK07B01) 公安部重点科技攻关项目(20034421401)
【分类号】：D919

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