气相色谱质谱联用法检测大鼠尿液中2C-B及其代谢物

发布时间：2018-07-15 15:41

【摘要】：目的研究4-溴-2,5-二甲氧基苯乙胺(2C-B)在大鼠体内的代谢物以及代谢途径。方法取W istar大鼠3只,以2C-B灌胃,收集24h内尿液,用β葡萄糖醛酸酶水解,Oasis(HLB柱固相提取,DB-35MS柱分析,气相色谱质谱联用检测。结果从大鼠尿液中检出6种2C-B的代谢产物,分别为:4-溴-2-羟基-5-甲氧基苯乙醇、4-溴-2,5-二甲氧基苯乙醇、4-溴-2-羟基-5-甲氧基苯乙酸、4-溴-2,5-二甲氧基苯乙酸、1-乙酰氨基-2-(4-溴-5-羟基-2-甲氧基苯)乙烷和1-乙酰氨基-2-(4-溴-2-羟基-5-甲氧基苯)乙烷。未检出2C-B原药。结论2C-B在大鼠尿液中主要以代谢物形式存在,其在大鼠体内至少存在两种代谢途径:第一种是2C-B的2位和5位氧上去甲基后氨基被乙酰化;第二种是2C-B去氨基生成醛,接着被还原或氧化生成醇和羧酸。
[Abstract]:Aim to study the metabolites and metabolic pathways of 4-bromo-2o-5-dimethoxyphenylethylamine (2-C-B) in rats. Methods three W istar rats were gavaged with 2C-B, and urine was collected within 24 hours. Oasis was hydrolyzed by 尾 -glucuronidase (HLB) with solid phase extraction (DB-35MS) column, and detected by gas chromatography-mass spectrometry (GC-MS). Results six metabolites of 2C-B were detected from rat urine. The results are as follows: 4- bromo-2-hydroxy-5-methoxy phenylethanol 4- bromo-2-hydroxy-5-methoxyphenylacetic acid 4- bromo-2-hydroxy-5-methoxy phenylacetic acid 4- (4-bromo-5-hydroxy-2-methoxyphenyl) ethane and 1-acetyl aminoethane (4-bromo-5-hydroxy-2-methoxy phenyl) ethane, 4-bromo-2-hydroxy-5-methoxy phenylacetic acid, 4-bromo-2-hydroxy-5-methoxyphenylacetic acid, 4-bromo-2-hydroxy-5-methoxy phenylacetic acid Group-2-(4-bromo-2-hydroxyl-5-methoxy-phenyl) ethane. 2C-B was not detected. Conclusion 2C-B exists mainly in the form of metabolites in the urine of rats. There are at least two metabolic pathways in rats: the first is acetylation of 2 and 5 sites of 2C-B, and the second is the formation of 2C-B deaminaldehyde. They are then reduced or oxidized to alcohols and carboxylic acids.
【作者单位】：广西壮族自治区公安厅物证鉴定中心;广西大学化学化工学院;广西壮族自治区公安厅科技处;
【基金】：公安部应用创新计划资助项目(2007YYCXGXST081) 广西壮族自治区公安厅科学研究与技术开发资助项目(200712)
【分类号】：D919

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