诺米芬新对亚急性甲基苯丙胺中毒大鼠多巴胺能神经系统的保护作用
发布时间：2018-08-17 19:02
【摘要】： 目的:探索诺米芬新对亚急性甲基苯丙胺中毒大鼠多巴胺能神经系统的保护作用并探讨其作用机制。 方法:Wistar雄性大鼠72只,随机分为生理盐水组(阴性对照组)、MA组(阳性对照组)和MA、Nomifensine组(保护药物组)三组。每晚8时分别腹腔注射Nacl 10mg/kg、MA 10mg/kg和MA 10mg/kg加Nom 2mg/kg,连续四天建立亚急性甲基苯丙胺中毒大鼠模型。通过观察动物一般情况、刻板行为和CPP实验,检验亚急性甲基苯丙胺中毒大鼠模型建立是否成功。在最后一次给药后的第一天、第七天和第十四天分别处死各组实验动物,用免疫组织化学染色法(S—P法)和荧光分光光度计法从形态学、半定量和定量的方法检测大鼠脑内黑质、中脑腹侧被盖区、前额叶皮质、海马、纹状体、伏隔核等部位多巴胺神经元细胞的形态和数量的变化。用图像分析系统对染色阳性神经细胞进行计数统计,对神经纤维进行灰度值分析,将各组数据结果进行比较及统计学处理。 结果:1.阴性对照组分别与阳性对照组、保护药物组在刻板行为评分和持续时间上比较呈显著性差异(P＜0.01);保护药物组和阳性对照组在刻板行为评分和持续时间上比较呈显著性差异(P分别为＜0.05和＜0.01);保护药物组和阳性对照组在CPP实验结果上比较呈显著性差异(P＜0.05)。2.不同脑区、不同时间点通过对神经细胞计数、神经纤维灰度值和TH含量荧光分光光度计测量,结果显示保护药物组神经系统损伤轻于阳性对照组。 结论:诺米芬新对亚急性甲基苯丙胺中毒大鼠多巴胺能神经系统具有保护作用。
[Abstract]:Aim: to explore the protective effect of Normifen on dopaminergic nervous system in rats with subacute methamphetamine poisoning and its mechanism. Methods Seventy-two male Wistar rats were randomly divided into three groups: normal saline group (negative control group) and MA-Nomifensine group (positive control group). Nacl 10 mg / kg MA 10mg/kg and MA 10mg/kg plus Nom 2 mg / kg were injected intraperitoneally every night at 8: 00 to establish subacute methamphetamine poisoning rat model for four days. The establishment of subacute methamphetamine poisoning rat model was tested by observing animal general situation, stereotypical behavior and CPP experiment. On the first day, the seventh day and the fourteenth day after the last administration of the drug, the experimental animals were killed, and the morphology of each group was studied by immunohistochemical staining (S-P method) and fluorescence spectrophotometer. The morphologic and quantitative changes of dopamine neurons in substantia nigra ventral tegmental area prefrontal cortex hippocampus striatum and nucleus accumbens were detected by semi-quantitative and quantitative methods. The positive nerve cells were counted and counted by image analysis system, the nerve fibers were analyzed by gray value, the results of each group were compared and statistically processed. The result is 1: 1. Negative control group and positive control group, There were significant differences in the scores and duration of stereotypic behavior between the protective drug group and the positive control group (P < 0.01), while there were significant differences between the protective drug group and the positive control group (P < 0.05 and P < 0.01 respectively). There was a significant difference between the protective drug group and the positive control group in CPP test results (P < 0. 05). 2. The nerve cell count, nerve fiber gray value and th content were measured by fluorescence spectrophotometer in different brain regions and at different time points. The results showed that the nerve system damage in the protective drug group was less than that in the positive control group. Conclusion: Normifen has protective effect on dopaminergic nervous system in rats with subacute methamphetamine poisoning.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：D919
本文编号：2188592
[Abstract]:Aim: to explore the protective effect of Normifen on dopaminergic nervous system in rats with subacute methamphetamine poisoning and its mechanism. Methods Seventy-two male Wistar rats were randomly divided into three groups: normal saline group (negative control group) and MA-Nomifensine group (positive control group). Nacl 10 mg / kg MA 10mg/kg and MA 10mg/kg plus Nom 2 mg / kg were injected intraperitoneally every night at 8: 00 to establish subacute methamphetamine poisoning rat model for four days. The establishment of subacute methamphetamine poisoning rat model was tested by observing animal general situation, stereotypical behavior and CPP experiment. On the first day, the seventh day and the fourteenth day after the last administration of the drug, the experimental animals were killed, and the morphology of each group was studied by immunohistochemical staining (S-P method) and fluorescence spectrophotometer. The morphologic and quantitative changes of dopamine neurons in substantia nigra ventral tegmental area prefrontal cortex hippocampus striatum and nucleus accumbens were detected by semi-quantitative and quantitative methods. The positive nerve cells were counted and counted by image analysis system, the nerve fibers were analyzed by gray value, the results of each group were compared and statistically processed. The result is 1: 1. Negative control group and positive control group, There were significant differences in the scores and duration of stereotypic behavior between the protective drug group and the positive control group (P < 0.01), while there were significant differences between the protective drug group and the positive control group (P < 0.05 and P < 0.01 respectively). There was a significant difference between the protective drug group and the positive control group in CPP test results (P < 0. 05). 2. The nerve cell count, nerve fiber gray value and th content were measured by fluorescence spectrophotometer in different brain regions and at different time points. The results showed that the nerve system damage in the protective drug group was less than that in the positive control group. Conclusion: Normifen has protective effect on dopaminergic nervous system in rats with subacute methamphetamine poisoning.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：D919
【参考文献】
相关期刊论文 前1条
1 周媛,邢淑华;苯丙胺CPP重现实验中c-fos和p-CREB在大鼠纹状体表达的变化[J];中国药物依赖性杂志;2005年03期
，本文编号：2188592
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