SD大鼠肢体挫伤后血清生化指标变化及其法医学意义

发布时间：2018-08-18 17:22

【摘要】：目的观察大鼠肢体挫伤后血清生化指标的变化特征,分析其法医学意义。方法实验选用SD大鼠,用自由落体装置分别击打大鼠右侧大腿根部,按垂直打击高度控制力度,设置为对照组、轻度打击组、中度打击组和重度打击组等4组。取实验鼠血清检测肌酸激酶(CK)、肌酸激酶同功酶(CK-Mb)、乳酸脱氢酶(LDH)、肌酐(Cr)、尿素氮(BUN)、α-羟丁酸脱氢酶(HBDH)及天门冬氨酸转移酶(AST)的变化,使用SPSS 12.0软件分析各指标变化趋势。结果7种血清生化指标AST、CK、CK-MB、HBDH、BUN、Cr随打击力度增加而上升,其中轻、中、重打击组作为整体损伤组与对照组比较有显著性差异,AST、CK、CK-Mb在打击组间比较具有显著性差异。打击组肢体周径与未打击组肢体周径比较有明显变化,且与打击力度呈线性相关。结论肢体软组织挫伤造成相关血清生化指标的显著变化,提示了发生早期心功能损害的部分机制,并且其损伤程度与打击力度呈现线性关系。
[Abstract]:Objective to observe the changes of serum biochemical indexes after limb contusion in rats and analyze their forensic significance. Methods Sprague-Dawley rats were randomly divided into 4 groups: control group, light strike group, moderate strike group and severe hit group. The changes of creatine kinase (CK), creatine kinase isoenzyme (CK-Mb), lactate dehydrogenase (LDH),) creatinine (Cr), urea nitrogen (BUN), 伪 -hydroxybutyrate dehydrogenase (HBDH) and aspartate transferase (AST) (AST) were detected in the serum of experimental rats. The change trend of each index was analyzed by SPSS 12.0 software. Results compared with the control group, there were significant differences among the seven serum biochemical indexes ASTX CKCK-MBN HBDHHUN-BUN-Cr in the light, middle and heavy attack groups as compared with the control group. There was significant difference between the two groups in the level of CK-Mb and CK-Mb. The circumference of the limbs in the strike group was significantly different from that in the control group, and was linearly correlated with the strike intensity. Conclusion there are significant changes in serum biochemical indexes related to soft tissue contusion of extremities, indicating some mechanisms of early cardiac function damage, and the degree of injury is linearly related to the intensity of attack.
【作者单位】：宁波市公安局;深圳市公安局南山分局;
【基金】：国家自然科学基金资助项目(30471935) 陕西省自然科学基金资助项目(2006C244-1)
【分类号】：D919

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