家兔急性有机磷中毒死后玻璃体液化学成分的变化趋势

发布时间：2018-09-10 18:18

【摘要】：目的探讨家兔急性有机磷中毒死后不同时间眼玻璃体液化学成分的变化。方法用5%敌百虫制作家兔急性有机磷中毒动物模型,在死后0~72h内不同时间点,采用留置微量重复取样法吸取眼玻璃体液,以全自动生化分析仪测量眼玻璃体液中钾、镁、磷、钙、钠、氯浓度的变化,统计数据进行二项式回归方程拟合分析。结果家兔急性有机磷中毒死后72h眼玻璃体液中钾、镁、磷随PM I延长而逐渐升高,R2分别为0.981 8、0.970 0、0.945 8,与PM I相关性好,其中30h内镁的相关性更好;钙、钠随PM I延长呈降低趋势,R2分别为0.712 4、0.735 5,相关性较差;氯表现不稳定R2仅为0.086 9。结论家兔急性有机磷中毒死后玻璃体液中钾、镁、磷、钙、钠等离子浓度随PM I延长而改变,72h内总体衡量,钾与PM I最具有相关关系。
[Abstract]:Objective to study the changes of vitreous humoral chemical composition in rabbits with acute organophosphorus poisoning at different time after death. Methods the animal model of acute organophosphorus poisoning in rabbits was made with 5% trichlorfon. At different time points within 72 hours after death, the vitreous fluid was extracted by indwelling microsampling method, and potassium, magnesium and phosphorus in ocular glass fluid were measured by automatic biochemical analyzer. The changes of calcium, sodium and chlorine concentrations were analyzed by binomial regression equation. Results after acute organophosphorus poisoning, potassium, magnesium and phosphorus in vitreous body fluid increased gradually with the prolongation of PM I, and the R2 was 0.981 ~ 0.9700 ~ 0.945 8, respectively, which had a good correlation with PM I, especially magnesium in 30 h. With the prolongation of PM I, the R2 of sodium decreased with the prolongation of PM I, and the R 2 was 0.712 4 卤0.735 5, respectively, the correlation was poor, and the unstable R2 of chlorine was only 0.086 9. Conclusion the plasma concentrations of potassium, magnesium, phosphorus, calcium and sodium in vitreous body fluid of rabbits with acute organophosphorus poisoning were changed with the prolongation of PM I within 72 hours, and the correlation between potassium and PM I was the most significant.
【作者单位】：重庆医科大学基础医学院法医教研室;重庆市公安局交通管理局;
【分类号】：D919

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