大鼠皮肤MMP-2,MMP-9表达与损伤时间相关性的实验研究

发布时间：2018-09-14 19:54

【摘要】：目的:探讨创伤皮肤组织基质金属蛋白酶(Matrix metalloproteinase-s,MMPs)-2、9的表达与损伤时间的相关性,为损伤时间推断提供实验依据。方法:建立大鼠皮肤切创模型,应用免疫组织化学技术、图像分析技术检测不同损伤时间大鼠皮肤组织生前及死后MMP-2与MMP-9表达。结果:生前伤组大鼠损伤后皮肤组织内MMP-2与MMP-9在损伤区及损伤区周围皮肤组织中表达明显高于正常对照组(P0.05)。死后伤组皮肤组织中MMP-2与MMP-9表达与正常对照组比较无明显差异(P0.05)。生前伤组中MMP-2在损伤后0.5h可见在中性粒细胞中阳性表达,随时间进展表达逐渐增强,并可见在少量血管内皮细胞中阳性表达,12h后明显增多,3d达到高峰,维持到第5d后在炎细胞中表达显著减少,而在成纤维细胞,血管内皮细胞中表达增加,维持在一个相对稳定的水平,12～14d恢复正常。MMP-9在损伤后1h可见在中性粒细胞中阳性表达,随时间进展表达逐渐增强,12h后明显增多,3d达到高峰,维持到第5d,5d后在炎细胞中表达显著减少,而在成纤维细胞,血管内皮细胞中表达增加,维持在一个相对稳定的水平,12～14d恢复正常。结论:皮肤损伤组织MMP-2与MMP-9表达具有规律性且有良好的时间相关性,可望成为法医学损伤时间推断的指标。
[Abstract]:Aim: to investigate the relationship between the expression of matrix metalloproteinase (Matrix metalloproteinase-s,MMPs)-2 and injury time in traumatic skin and to provide experimental evidence for estimating injury time. Methods: the rat skin injury model was established and the expression of MMP-2 and MMP-9 was detected by immunohistochemistry and image analysis before and after death. Results: the expression of MMP-2 and MMP-9 in the skin tissue of the injured group was significantly higher than that of the normal control group (P0.05). There was no significant difference in the expression of MMP-2 and MMP-9 between postmortem injury group and normal control group (P0.05). Positive expression of MMP-2 was observed in neutrophil at 0.5 h after injury, and increased gradually with time, and increased significantly in a small number of vascular endothelial cells at 12 h after injury. The expression of MMP-9 was significantly decreased in inflammatory cells at the 5th day, but increased in fibroblasts. The positive expression of MMP-9 was found in neutrophils 1 h after injury at a relatively stable level of 12d to 14d, and the positive expression of MMP-9 was found in neutrophilic granulocytes at 1 hour after injury, and the positive expression of MMP-9 was found in neutrophils 1 h after injury. After 12 hours, the expression increased to the peak at the 3rd day, decreased significantly in the inflammatory cells after 5 days, but increased in the fibroblasts and vascular endothelial cells. It was maintained at a relatively stable level and recovered to normal at 12 ~ 14 d. Conclusion: the expression of MMP-2 and MMP-9 in skin injury tissues is regular and has a good time correlation, which is expected to be an index for estimating the time of injury in forensic medicine.
【作者单位】：重庆医科大学基础医学院法医学教研室;重庆医科大学附属第一医院中西医结合科;
【分类号】：D919.2

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