类胰蛋白酶和IgE测定在过敏性猝死诊断中的应用研究

发布时间：2018-10-26 19:11

【摘要】：目的探讨过敏性猝死法医学鉴定的诊断方法和指标。方法采取10例正常人、9例过敏性猝死和19例其他死因(排除过敏反应、冠心病)尸体的静脉血,采用荧光酶联免疫法(Pharmacia UniCAP100过敏原定量分析仪)和酶联免疫吸附试验ELISA法分别测定血清肥大细胞类胰蛋白酶和IgE含量,采用免疫组化方法观察过敏性猝死和其他死因的肺组织中的肥大细胞类胰蛋白酶免疫组化染色。结果过敏性猝死者的血清类胰蛋白酶和IgE含量升高,与其他死因之间的差异具有显著性意义(P0.01),其他死因和正常人之间的差异无统计学意义(P(0.05);与其它死因相比,过敏性猝死肺组织中的肥大细胞类胰蛋白酶免疫组化阳性染色增强(P0.01)。结论过敏性猝死者血清IgE和肥大细胞类胰蛋白酶含量显著升高;过敏性猝死者肺组织中肥大细胞类胰蛋白酶染色增强。
[Abstract]:Objective to investigate the diagnostic methods and indicators of anaphylactic sudden death in forensic medicine. Methods the venous blood of 10 normal subjects, 9 cases of sudden anaphylactic death and 19 cases of other causes of death (excluding allergic reaction, coronary heart disease) were collected. The levels of mast cell trypsin and IgE in serum were measured by fluorescence enzyme-linked immunosorbent assay (Pharmacia UniCAP100) and enzyme-linked immunosorbent assay (ELISA), respectively. Immunohistochemical staining of mast cell trypsin-like trypsin in lung tissues of sudden allergic death and other causes of death was observed by immunohistochemical method. Results the levels of serum trypsin and IgE in patients with sudden anaphylactic death were significantly higher than those in other causes of death (P0.01), but there was no significant difference between other causes of death and normal persons (P0.01). Compared with other causes of death, the mast cell trypsin positive staining was enhanced in the lung tissue of sudden allergic death (P0.01). Conclusion the levels of serum IgE and mast cell trypsin were significantly increased in patients with sudden anaphylactic death and the staining of mast cell trypsin was enhanced in the lung tissues of patients with sudden allergic death.
【作者单位】：山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室山西医科大学法医学院法医病理学教研室
【基金】：山西省自然基金项目(20051090) 山西省科技攻关项目(2006031087-06)
【分类号】：D919

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