KCNQ1内含子1a中STR基因座遗传多态性及PIA分型
发布时间：2018-11-02 16:11
【摘要】：目的调查汉族群体KCNQ1基因内含子1a中STR基因座的遗传多态性,并采用PIA分型技术确定等位基因的亲代来源。方法用PCR-STR分型技术对230例武汉汉族无关个体样本进行KCNQ1基因内含子1a中STR基因座分型检测;同时选用两种甲基化敏感的限制酶(msRE)HhaⅠ和HpaⅡ对家系中孩子的基因组DNA进行消化后,采用PIA分型技术检测父源等位基因。结果KCNQ1内含子1a中STR基因座在汉族人群中检出10个等位基因、24种基因型,其个体识别能力(PD)、多态性信息含量(PIC)和非父排除率(PE)分别为0.852、0.66和0.484。HhaⅠ和HpaⅡ可消化个体的母源等位基因,PIA分型仅能检测出单一的父源等位基因。结论KCNQ1内含子1a中STR基因座在汉族群体具有较高的遗传多态性,PIA分型技术可以确定个体等位基因的亲代来源,具有较高的法医学应用价值。
[Abstract]:Objective to investigate the genetic polymorphism of STR locus in intron 1a of KCNQ1 gene in Han population, and to determine the source of allele by PIA typing. Methods the STR loci in intron 1a of KCNQ1 gene were detected by PCR-STR typing in 230 unrelated individuals of Wuhan Han nationality. At the same time, two methylation-sensitive restriction enzymes, (msRE) Hha 鈪，
本文编号：2306289
[Abstract]:Objective to investigate the genetic polymorphism of STR locus in intron 1a of KCNQ1 gene in Han population, and to determine the source of allele by PIA typing. Methods the STR loci in intron 1a of KCNQ1 gene were detected by PCR-STR typing in 230 unrelated individuals of Wuhan Han nationality. At the same time, two methylation-sensitive restriction enzymes, (msRE) Hha 鈪，
本文编号：2306289
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