过度机械通气致肺损伤TNF-α、IL-1β及NF-κBp65的表达

发布时间：2018-11-08 06:38

【摘要】：目的探讨过度机械通气致肺损伤后,炎症因子TNF-α、IL-1β及NF-κBp65在肺组织中不同时间表达的变化规律。方法将51只雄性新西兰大白兔分为正常组、对照组、实验组。利用呼吸机建立过度机械通气致肺损伤模型。应用免疫印迹技术观察兔急性肺损伤后肺组织中TNF-α、IL-1β及NF-κBp65的表达,并用凝胶图像处理系统进行分析,所得数据经SPSS 10.0统计软件处理。结果正常组与各对照组之间比较,均无明显差异(P0.05)。TNF-α在损伤后0h表达即增加,1h达高峰,以后逐渐下降,而在24h后又有反弹性增高;其中在0.5、24、48h组有差异性(P0.05),1、3h组有显著差异性(P0.01)。IL-1β在损伤后0.5h逐渐增加,3h达高峰,以后逐渐下降,其中在1、6、12、24h组有差异性(P0.05),3h组有显著差异性(P0.01)。NF-κBp65在损伤后0.5h逐渐增加,6h达高峰,以后逐渐下降,其中在1、12h组有差异性(P0.05),3、6h组有显著性差异(P0.01)。结论TNF-α、IL-1β及NF-κBp65在过度机械通气致VILI发病中有重要作用;TNF-α、IL-1β及NF-κBp65在损伤早期即表达,且在时间上有一定的变化规律,可为损伤时间的推断提供参考。
[Abstract]:Objective to investigate the expression of inflammatory factors TNF- 伪, IL-1 尾 and NF- 魏 Bp65 in lung tissue after lung injury induced by hyperventilation. Methods 51 male New Zealand rabbits were divided into normal group, control group and experimental group. The model of lung injury induced by hyperventilation was established by ventilator. The expressions of TNF- 伪, IL-1 尾 and NF- 魏 Bp65 in rabbit lung tissue after acute lung injury were observed by Western blotting technique. The data were analyzed by gel image processing system. The data were processed by SPSS 10.0 statistical software. Results there was no significant difference between the normal group and the control group (P0.05). The expression of TNF- 伪 increased at 0 h after injury, reached the peak at 1 h, then decreased gradually, and then rebounded after 24 h. IL-1 尾 increased at 0.5 h after injury, peaked at 3 h after injury, then decreased gradually (P0.05), and there was significant difference in 1h group (P0.05). NF- 魏 Bp65 increased gradually at 0.5 h after injury, peaked at 6 h, and then decreased gradually (P0.05) in 1h / 12h group and significantly (P0.01) in 3h group (P0.01). Conclusion TNF- 伪, IL-1 尾 and NF- 魏 Bp65 play an important role in the pathogenesis of VILI induced by hyperventilation. TNF- 伪, IL-1 尾 and NF- 魏 Bp65 were expressed at the early stage of injury, and there were some changes in time, which could provide reference for the estimation of injury time.
【作者单位】：湖南省第二人民医院综合司法鉴定室;湖南中医药大学病理教研室;
【分类号】：D919

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