小鼠皮肤损伤修复过程中白细胞介素1α和白细胞介素1β变化与损伤时间的关系

发布时间：2018-11-09 12:30

【摘要】：目的:研究表明,细胞因子在各种炎症反应、损伤修复及细胞突变过程中发挥着重要作用,并且有望成为推断损伤时间新的有效指标。实验拟验证皮肤损伤后损伤区组织内不同时间白细胞介素1α和白细胞介素1β基因表达的变化及其与损伤时间的关系。方法:实验于2006-03/2007-12在中国刑警学院法医系实验室完成。①实验材料:清洁级BALB/c纯系雄性小白鼠35只,体质量25~27g。②实验方法:建立动物皮肤损伤模型,在脊柱两侧1.0cm处,左右对称各做一处皮肤缺损损伤,损伤不伤及肌肉,每只鼠做3对损伤。损伤后分别在0,3,6,12h,1,3,6d(0h作为对照)取5只动物麻醉断头处死。③实验评估:免疫组织化学染色后,显微镜下观察不同时间点白细胞的变化,实时定量聚合酶链反应检测白细胞介素1α和白细胞介素1β两种炎症因子的基因表达。结果:纳入BALB/c纯系雄性小白鼠35只,均进入结果分析。①伤后0~5h,皮肤创缘呈弱阳性反应;伤后6~24h,表皮阳性反应减弱,创伤局部以巨噬细胞为主的阳性细胞逐渐增多。伤后1d可见嗜中性白细胞大量浸润及少量单核细胞;伤后2~4d,单核细胞数目逐渐增加,3d达高峰。对照组皮肤表皮、毛囊、皮脂腺和汗腺均呈弱阳性反应。②皮肤损伤后在早期可诱导白细胞介素1α和白细胞介素1β的基因表达,6h达第一次高峰,24h下降,第3天再次出现高峰,第6天下降至正常水平。结论:损伤组织中白细胞介素1α和白细胞介素1β的基因表达随伤后不同时间呈现一定规律性变化,可为法医学案例早期损伤时间的推断提供参考。
[Abstract]:Objective: the study shows that cytokines play an important role in various inflammatory reactions, injury repair and cell mutation, and may become a new effective index for estimating injury time. The purpose of this study was to verify the changes of IL-1 伪 and IL-1 尾 gene expression and the relationship between the expression of IL-1 伪 and IL-1 尾 gene and injury time after skin injury. Methods: the experiment was carried out in the Laboratory of Forensic Medicine Department of China Criminal Police College in December 2006 and December 2007. 1 Experimental materials: 35 pure male mice of clean grade BALB/c. Body mass 25~27g.2 test method: establish animal skin injury model. At the 1.0cm on both sides of the spine, the left and right symmetrical skin defect injury was done, without injury to the muscle, and 3 pairs of injury were done in each mouse. After injury, 5 animals were anesthetized for 6 days (0 h as control). 3 Experimental evaluation: after immunohistochemical staining, the changes of white blood cells at different time points were observed under microscope. Real-time quantitative polymerase chain reaction (PCR) was used to detect the gene expression of IL 1 伪 and IL 1 尾. Results: 35 male BALB/c mice were involved in the analysis of the results. 1 the skin wound edge was weakly positive at 5 h after injury, and the epidermal positive reaction was weakened at 6 and 24 h after injury, and the number of macrophage positive cells increased gradually. A large number of neutrophil infiltration and a small number of monocytes were observed on the 1st day after injury, and the number of monocytes increased gradually on the 2nd day after injury and reached the peak on the 3rd day after injury. The skin epidermis, hair follicle, sebaceous gland and sweat gland of the control group were weakly positive. 2 the gene expression of IL-1 伪 and IL-1 尾 could be induced at the early stage of skin injury, and reached the first peak at 6 h and decreased at 24 h. On the third day there was a peak again, and on the sixth day it fell to a normal level. Conclusion: the gene expression of IL-1 伪 and IL-1 尾 in injured tissues changes regularly with different time after injury, which can provide reference for early injury time in forensic cases.
【作者单位】：中国刑事警察学院法医学系沈阳医学院基础医学部生物化学与分子生物学教研室
【分类号】：D919

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