大鼠电流损伤后心肌PEG-3表达的法医学研究

发布时间：2019-01-04 15:19

【摘要】：目的:探讨电击伤和电击死后心肌中PEG-3表达的时间相关性以及其能否用于生前电击和死后电击的鉴别。方法:建立大鼠电流损伤的模型。电击伤组于电击后0h、3h、6h、12h、24h、3d、6d、12d断颈处死取材;电击死组分别于死后0h、1h、3h、6h、12h取材;死后电击组分别于断颈处死后的0h、0.5h、1h、3h电击后取材;阴性对照组直接断颈处死后分别于0h、0.5h、1h、3h、6h、12h取材,取材的部位均为左心室前壁中央部,取材后采用免疫组化染色法检测所有大鼠心肌PEG-3的表达情况,其结果经图象分析处理后进行统计分析。结果:电击伤(死)组在电损伤早期PEG-3即表达增加,且不同时间的表达有一定的规律,故可用于推断心肌电击伤(死)的时间。而在死后电击及阴性对照组只有弱表达,根据这点可以将死后电击和电击致死进行区分。结论:心肌电损伤后PEG-3的表达有一定的时间相关性,可以用于对电击伤(死)的诊断;心肌电损伤后PEG-3的表达情况可以鉴别生前电击和死后电击。
[Abstract]:Aim: to investigate the temporal correlation of PEG-3 expression in myocardium between electrocution and postmortem injury and whether it can be used to differentiate between antemortem shock and postmortem shock. Methods: the rat model of current injury was established. The rats in the electric shock group were killed and killed at 0 h, 3 h, 6 h, 12 h, 24 h, 6 d, 6 d, 12 d, respectively, and the electrocution group were collected at 0 h, 1 h, 3 h, 6 h and 12 h, respectively. The postmortem shock group was taken at 0 h, 0.5 h, 1 h and 3 h after cervical amputation. In the negative control group, the specimens were obtained from the central part of the anterior wall of the left ventricle at 0 h, 0.5 h, 1 h, 3 h and 6 h and 12 h, respectively. The expression of PEG-3 in the myocardium of all the rats was detected by immunohistochemical staining. The results were statistically analyzed after image analysis. Results: the expression of PEG-3 increased at the early stage of electrical injury, and the expression of PEG-3 was regular at different time, so it could be used to infer the time of myocardial electrical injury (death). There was only a weak expression in postmortem shock and negative control, according to which postmortem shock and electrocution could be distinguished. Conclusion: the expression of PEG-3 after myocardial electrical injury is time-dependent and can be used in the diagnosis of electrical injury (death), and the expression of PEG-3 after myocardial electrical injury can be distinguished between antemortem and postmortem electric shock.
【作者单位】：重庆医科大学基础医学院法医学教研室;汉中市3201医院病理科;
【分类号】：D919

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