甲基苯丙胺在家兔体内死后再分布规律的研究
发布时间：2019-05-17 20:42
【摘要】：死后再分布(postmortem redistribution PMR)是指死后体内毒(药)物在组织、器官以及体液间发生移动的现象。在机体死后不久毒(药)物的扩散作用就开始了,以致于死后不同时间不同组织、器官内的毒药物浓度不尽相同,传统的检材选取方法可靠性值得怀疑。PMR的发生机制十分复杂,目前提出的主要有:梯度浓度扩散,死后血液的流动,体内药物代谢动力学作用,腐败作用等。死后再分布的机制十分复杂,同一种毒物中毒,在不同的体液及组织器官内PMR可能同时涉及多种机制。由于甲基苯丙胺(Methamphetamine MA)滥用现象极为严重,中毒及致死的案件时有发生,根据其化学性质推断,理论上MA存在PMR的可能,因此本课题选取MA做研究对象。 目的拟建立生物检材中MA快速、灵敏的提取、检测方法,研究MA在家兔体内是否存在PMR现象,并阐述其PMR的变化趋势,为此类毒品中毒案件的法医学检验鉴定提供理论依据和有效检验方法。 方法选择30只实验家兔分6组,以MA溶液按照30mg/kg的量灌胃1~4组、以60mg/ml的MA乙醇溶液按照30mg/kg的量液灌胃5、6组,以窒息方式处死后以仰卧位置于15℃环境内,分别于死后0h、6h、24h、48h取心血、外周血、尿液及肺组织、肝组织、脑组织待测1~4组,于死后6h、48h检验5、6组。 以SPME-GC-MS法检测家兔不同体液及组织中的MA,使用100μm聚二甲基硅氧烷萃取头萃取,1-萘胺(1-Naphthylamine)作为内标,以气相色谱质谱选择离子法进行检测。所得数据用SPSS13.0软件进行方差分析和多样本均数多重比较的t检验。 结果MA在急性中毒家兔体内存在PMR现象,家兔死亡后0h~48h内心血、外周血、肝组织内MA浓度逐渐出现不同程度的升高(P0.01),肺组织内浓度先升高后下降(P0.05),尿液内的浓度轻度下降(P0.05),脑组织浓度变化较小(P0.05),死后早期浓度变化较晚期明显。MA合并乙醇组家兔死亡后6h、48hMA在急性中毒家兔心血、肝组织中有升高趋势(P0.05),PMR现象较单独使用MA时明显增强。 结论 1.建立了生物检材中MA的SPME-GC-MS快速提取、检测方法。 2.急性中毒家兔死后体液及组织中MA出现死后再分布现象,MA含量随死后时间的延长,出现不同程度死后再分布变化,脑组织浓度变化较小。 3.要准确地判断死亡当时的体液和组织中的MA含量,取材检测时应考虑死后再分布因素的影响。
[Abstract]:Postmortem redistribution of (postmortem redistribution PMR) refers to the movement of toxins between tissues, organs and body fluids after death. Soon after the death of the body, the diffusion of poison (drug) began, so that the concentration of toxic drugs in organs varied from tissue to tissue at different time after death. The reliability of traditional sample selection methods is questionable. The occurrence mechanism of PMR is very complex. At present, the main proposals are gradient concentration diffusion, postmortem blood flow, pharmacokinetics in vivo, corruption and so on. The mechanism of postmortem redistribution is very complex. PMR may involve many mechanisms in different body fluids and tissues and organs at the same time. Because the abuse of methamphetamine (Methamphetamine MA) is very serious, poisoning and death occur from time to time. According to its chemical properties, it is inferred that MA has the possibility of PMR in theory, so MA is selected as the research object in this paper. Objective to establish a rapid and sensitive method for the extraction and detection of MA from biological samples, to study the existence of PMR in MA in rabbits, and to explain the changing trend of PMR. It provides theoretical basis and effective test method for forensic examination and identification of this kind of drug poisoning cases. Methods 30 rabbits were divided into 6 groups. MA solution was given intragastrically according to the amount of 30mg/kg, MA ethanol solution of 60mg/ml was given intragastrically according to the dose of 30mg/kg for 5, 6 groups were killed by asphyxiation and lying on their backs at 15 鈩，
本文编号：2479390
[Abstract]:Postmortem redistribution of (postmortem redistribution PMR) refers to the movement of toxins between tissues, organs and body fluids after death. Soon after the death of the body, the diffusion of poison (drug) began, so that the concentration of toxic drugs in organs varied from tissue to tissue at different time after death. The reliability of traditional sample selection methods is questionable. The occurrence mechanism of PMR is very complex. At present, the main proposals are gradient concentration diffusion, postmortem blood flow, pharmacokinetics in vivo, corruption and so on. The mechanism of postmortem redistribution is very complex. PMR may involve many mechanisms in different body fluids and tissues and organs at the same time. Because the abuse of methamphetamine (Methamphetamine MA) is very serious, poisoning and death occur from time to time. According to its chemical properties, it is inferred that MA has the possibility of PMR in theory, so MA is selected as the research object in this paper. Objective to establish a rapid and sensitive method for the extraction and detection of MA from biological samples, to study the existence of PMR in MA in rabbits, and to explain the changing trend of PMR. It provides theoretical basis and effective test method for forensic examination and identification of this kind of drug poisoning cases. Methods 30 rabbits were divided into 6 groups. MA solution was given intragastrically according to the amount of 30mg/kg, MA ethanol solution of 60mg/ml was given intragastrically according to the dose of 30mg/kg for 5, 6 groups were killed by asphyxiation and lying on their backs at 15 鈩，
本文编号：2479390
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