外力打击兔颈动脉窦压力感受器引起动物死亡心肌组织ANP及血浆儿茶酚胺含量变化规律的研究
发布时间：2019-07-08 20:00
【摘要】： 研究目的:观察外力打击兔颈动脉窦压力感受器后引起动物死亡,观察组组织形态学变化和分析测定心肌组织ANP及血浆儿茶酚胺含量的差异,探讨引起死亡的机制。 方法:机械性外力打击兔颈动脉窦压力感受器后造成动物神经源性休克死亡模型,观察组织形态学的变化,运用Western blot检测心肌组织ANP水平,高效液相-电化学法(HPLC-ECD)检测儿茶酚胺含量,并与对照组比较。 结果:1、形态学研究:实验组死亡动物经过检验未发现器质性病变,其死亡均由颈动脉窦压力感受器被打击后所致。形态学观察发现颈动脉窦外膜损伤表现为灶性片状出血、神经纤维断裂、神经纤维轴浆溶解;心肌组织表现为急性缺血性损伤改变;无明显肺水肿表现。而存活组亦有部分颈动脉外膜灶性出血、轻度急性心肌缺血性损伤改变。 2、与对照组与存活组比较,死亡组右心耳ANP含量较低。 3、死亡组血浆儿茶酚胺较对照组含量明显增高(3-10倍)。 结论:颈动脉窦压力感受器受外界压力刺激引起死亡的改变有:颈动脉窦外膜损伤表现为灶性片状出血、神经纤维断裂、神经纤维轴浆溶解,急性心肌缺血性损伤改变;心肌组织ANP含量减少;血浆儿茶酚胺含量明显升高。
文内图片：
图片说明：兔颈内动脉打击部位外膜出血HE×20Fig-1haemorrhageofrabbit’sinternalcarotidarteryadventitiabyhit(HE10×20)
[Abstract]:Objective: to observe the animal death caused by external force striking carotid sinus baroreceptor in rabbits, to observe the morphological changes of myocardial tissue and the difference of plasma catecholamine content in myocardial tissue, and to explore the mechanism of death. Methods: the animal model of neurogenic shock was established after mechanical external force hit the carotid sinus baroreceptor in rabbits. The histomorphological changes were observed. The level of ANP in myocardial tissue was measured by Western blot, and the content of catecholamine was detected by high performance liquid phase electrochemical method (HPLC-ECD) and compared with the control group. Results: 1. Morphological study: no organic lesions were found in the dead animals in the experimental group, and the deaths were caused by the attack of carotid sinus baroreceptor. Morphological observation showed that the injury of carotid sinus adventitia showed focal flake hemorrhage, broken nerve fibers and axonal plasma dissolution of nerve fibers, acute ischemic injury in myocardial tissue and no obvious pulmonary edema. In the survival group, some focal hemorrhage of carotid artery and mild acute myocardial ischemic injury were also found. 2. Compared with the control group and survival group, the ANP content of right atrial appendage in the death group was lower than that in the control group and survival group. 3. The content of plasma catecholamine in the dead group was significantly higher than that in the control group (3 鈮，
本文编号：2511837
文内图片：
图片说明：兔颈内动脉打击部位外膜出血HE×20Fig-1haemorrhageofrabbit’sinternalcarotidarteryadventitiabyhit(HE10×20)
[Abstract]:Objective: to observe the animal death caused by external force striking carotid sinus baroreceptor in rabbits, to observe the morphological changes of myocardial tissue and the difference of plasma catecholamine content in myocardial tissue, and to explore the mechanism of death. Methods: the animal model of neurogenic shock was established after mechanical external force hit the carotid sinus baroreceptor in rabbits. The histomorphological changes were observed. The level of ANP in myocardial tissue was measured by Western blot, and the content of catecholamine was detected by high performance liquid phase electrochemical method (HPLC-ECD) and compared with the control group. Results: 1. Morphological study: no organic lesions were found in the dead animals in the experimental group, and the deaths were caused by the attack of carotid sinus baroreceptor. Morphological observation showed that the injury of carotid sinus adventitia showed focal flake hemorrhage, broken nerve fibers and axonal plasma dissolution of nerve fibers, acute ischemic injury in myocardial tissue and no obvious pulmonary edema. In the survival group, some focal hemorrhage of carotid artery and mild acute myocardial ischemic injury were also found. 2. Compared with the control group and survival group, the ANP content of right atrial appendage in the death group was lower than that in the control group and survival group. 3. The content of plasma catecholamine in the dead group was significantly higher than that in the control group (3 鈮，
本文编号：2511837
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