Connectivity map技术在中药研究中的应用

发布时间：2018-03-30 06:36

  本文选题：Connectivity　切入点：Map技术　出处：《浙江大学学报(农业与生命科学版)》2016年05期

【摘要】：Connectivity map(CMap)是基于RNA芯片研究药物作用机制和药物重定位的技术与数据资源,主要的优势在于能够高效地对全基因组上的基因表达做整体分析。相对于低通量的药理学、毒理学方法以及色谱技术,该技术能以全面、高通量、准确等优点广泛应用于药物研究。尤其对由多种化合物共同作用发挥疗效的中药,CMap技术可以突破"药物-靶标"测定技术的单一性困难,对中药的作用机制做综合性分析,从分子水平上阐明中药的药物作用机制,有利于开发自主知识产权的中药,也为中药获取国际认可奠定理论基础。本文系统介绍CMap技术在中药研究方面的应用潜力,同时对该技术研究中药的作用机制、药物重定位及鉴别有效成分进行评价,还对推动中药走向国际市场做了具体分析与展望。
[Abstract]:Connectivity mapp is a technology and data resource based on RNA chip to study the mechanism of drug action and drug relocalization. Its main advantage lies in its ability to efficiently analyze gene expression in the whole genome, compared with low throughput pharmacology. Toxicological methods and chromatographic techniques that can be used in a comprehensive, high-throughput, The advantages of accuracy are widely used in drug research. In particular, the CMap technique, which has the effect of multiple compounds, can break through the difficulty of the "drug-target" determination technique, and make a comprehensive analysis of the mechanism of the action of traditional Chinese medicine. Elucidating the mechanism of drug action on molecular level is beneficial to the development of traditional Chinese medicine with independent intellectual property rights, and also lays a theoretical foundation for obtaining international recognition of Chinese medicine. This paper systematically introduces the application potential of CMap technology in the research of traditional Chinese medicine. At the same time, the mechanism, reorientation and identification of active components of traditional Chinese medicine were evaluated, and the specific analysis and prospect of promoting Chinese medicine to the international market were made.
【作者单位】： 昆明理工大学医学院疾病与药物遗传实验室;
【基金】：国家自然科学基金(81460007)
【分类号】：R28
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本文编号：1684754