LA百合核型分析及其与大花卷丹杂交亲和性研究
发布时间：2019-06-20 06:12
【摘要】：百合是单子叶植物亚纲百合科(Liliacea)百合属(Lilium)植物的总称,是世界重要的园艺观赏花卉。其花大色艳,姿态优美,品种繁多,是国内外花卉市场上五大鲜切花之一。同时,它也适于园林绿化与盆栽。我国有着丰富的百合资源,但在百合育种方面才刚起步。与国外规范化的百合育种成果相比缺乏具有自主知识产权的百合新品种。现代百合育种方向是将不同品种间优良性状整合为一体,获得新目标性状百合杂交品种。本试验拟对收集的9个LA百合杂种系进行倍性调查,同时对部分LA百合杂种系品种与野生大花卷丹Lilium leichtlinii var. maximowiczii Baker进行杂交育种,将野生种所具有的优良性状引入栽培品中,并对杂交组合亲和性进行分析与评价。主要研究结果如下：1.对LA百合杂种系9个品种进行倍性调查,利用常规压片技术对其进行核型分析。通过分析可知这9个LA杂交品种均为三倍体,其核型公式为：Cilesta':2n=3x=36 =6m+6sm(6SAT)+15st(6SAT)+9t; 'Couplet':2n=3x=36=6m+6sm(6SAT)+ 18st(12SAT)+6t;'Eyeliner':2n=3x=36:9m(3SAT)+3sm(3SAT)+18st(12SAT)+6t； 'Menorca':2n=3x=36=9m(3SAT)+6sm(6SAT)+12st(9SAT)+9t:'Serrada':2n.3x =36=6m(3SAT)+9sm(3SAT)+12st(6SAT)+9t；'Donau':2n=3x.36=9m(6SAT)+ 9sm(6SAT)+12St(3SAT)+6t；'Desiderio':2n=3x:36=9m(3SAT)+6sm+15st(9SAT) +6t(3SAT)；'Laskmi':2n=3x=36=9m(3SAT)+6sm(6SAT)+15st(6SAT)+6t:'White sourld':2n=3x=36=9m(3SAT)+9sm(9SAT)+15st(6SAT)+3t。其核不对称系数分别为：75.76%、76.33%、75.30%、73.69%、74.81%、71.89%、73.43%、73.10%、71.84%。2.以LA杂种系为母本,大花卷丹为父本进行杂交组合设计,共计13个杂交组合,杂交授粉花朵数为390朵,收获种子为3164粒。获得结实率高于25%的组合10个。3.利用荧光电子显微技术对大花卷丹自交授粉和LA杂种系的'California’×大花卷丹杂交授粉后的花粉管行为进行观察,LA与大花卷丹杂交存在一定障碍,大花卷丹的花粉可以在LA百合杂种系的柱头上萌发,但花粉萌发滞后,花粉管伸长速度慢,难以在48h内伸入子房到达胚囊完成受精。但杂交后仍可获得有胚种子。而大花卷丹自交,花粉萌发速度快,花粉管可以在48h-72h内伸入子房完成受精。
[Abstract]:Lily is a general name of (Lilium), a monocotyledonous plant, (Liliacea) Liliaceae. It is an important horticultural ornamental flower in the world. Its flowers are bright, beautiful posture, a wide variety, is one of the five fresh cut flowers in the flower market at home and abroad. At the same time, it is also suitable for landscaping and potting. China is rich in lily resources, but it has just started in lily breeding. Compared with the foreign standardized lily breeding results, there is a lack of new lily varieties with independent intellectual property rights. The breeding direction of modern lily is to integrate the excellent characters among different varieties and obtain the new target characters of lily hybrid varieties. In this experiment, the ploidy of 9 LA lily hybrid lines collected was investigated, and some LA lily hybrid lines and wild Dahuadan Lilium leichtlinii var. were also investigated. Maximowiczii Baker was used for cross breeding, and the excellent characters of wild species were introduced into the cultivated products, and the affinity of hybrid combinations was analyzed and evaluated. The main results are as follows: 1. The ploidy of 9 hybrid lines of LA lily was investigated and their karyotype was analyzed by conventional tablet pressing technique. The karyotype formula of the nine LA hybrid varieties was as follows: Cilesta':2n=3x=36 = 6m 6sm (6SAT) 15st (6SAT) 9t, 'Couplet':2n=3x=36=6m 6sm (6SAT) 18st (12SAT) 6t 鈮，
本文编号：2503001
[Abstract]:Lily is a general name of (Lilium), a monocotyledonous plant, (Liliacea) Liliaceae. It is an important horticultural ornamental flower in the world. Its flowers are bright, beautiful posture, a wide variety, is one of the five fresh cut flowers in the flower market at home and abroad. At the same time, it is also suitable for landscaping and potting. China is rich in lily resources, but it has just started in lily breeding. Compared with the foreign standardized lily breeding results, there is a lack of new lily varieties with independent intellectual property rights. The breeding direction of modern lily is to integrate the excellent characters among different varieties and obtain the new target characters of lily hybrid varieties. In this experiment, the ploidy of 9 LA lily hybrid lines collected was investigated, and some LA lily hybrid lines and wild Dahuadan Lilium leichtlinii var. were also investigated. Maximowiczii Baker was used for cross breeding, and the excellent characters of wild species were introduced into the cultivated products, and the affinity of hybrid combinations was analyzed and evaluated. The main results are as follows: 1. The ploidy of 9 hybrid lines of LA lily was investigated and their karyotype was analyzed by conventional tablet pressing technique. The karyotype formula of the nine LA hybrid varieties was as follows: Cilesta':2n=3x=36 = 6m 6sm (6SAT) 15st (6SAT) 9t, 'Couplet':2n=3x=36=6m 6sm (6SAT) 18st (12SAT) 6t 鈮，
本文编号：2503001
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